Isoptericola sediminis sp. nov., Isolated from Chilika Lagoon.

A yellow-colored, Gram-stain-positive, rod shaped, non-motile bacterium, designated as strain JC619, was isolated from the sediment of Chilika lagoon, India. Strain JC619 shows highest 16S rRNA gene sequence similarity (99.08%) with Isoptericola chiayiensis KCTC 19740 followed by Isoptericola halotolerans KCTC 19046 (98.6%) and other members of the genus Isoptericola (< 98%). NaCl is required for growth of strain JC619 and tolerates up to 18% (w/v) and pH up to 10. Strain JC619 grows optimally at temperature 30 °C, NaCl concentration of 3% (w/v), and at pH 7.5. The genome size of strain JC619 is 3.2 Mb with G+C content of 73.0 mol%. ANI scores of strain JC619 are 81.9% and 80.1% and dDDH values are 24.4% and 22.7% with I. chiayiensis KCTC 19740 and I. halotolerans KCTC 19046, respectively. Respiratory quinones are MK-9(H4) and MK-9(H2). Predominant fatty acids (> 10%) are anteiso-C, iso-C, and iso-C. Major polar lipids of strain JC619 are phosphatidylglycerol, phosphatidylinositolmannoside, diphosphatidylglycerol, and phosphatidylinositol. Strain JC619 is catalase positive but cytochrome oxidase negative and reduces nitrate. The genomic distinction of strain JC619 with its nearest related species of the genus Isoptericola is well supported with chemotaxonomic characteristics and differential physiological properties. Therefore, strain JC619 represents a new species under the genus Isoptericola for which Isoptericola sediminis sp. nov. is proposed. Type strain is JC619 (=KCTC 49244 =NBRC 114063).