肾脏 pH 值测绘的化学交换饱和传递（CEST）MRI：实验方案。
Renal pH Mapping Using Chemical Exchange Saturation Transfer (CEST) MRI: Experimental Protocol.
机构信息
Division of MR Research, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy.
出版信息
Methods Mol Biol. 2021;2216:455-471. doi: 10.1007/978-1-0716-0978-1_27.
Chemical exchange saturation transfer (CEST) is recognized as one of the premier methods for measuring pH with this environmental variable expected to be an excellent biomarker for kidney diseases. Here we describe step-by-step CEST MRI experimental protocols for producing pH and perfusion maps for monitoring kidney pH homeostasis in rodents after administering iopamidol as contrast agent. Several CEST techniques, acquisition protocols and ratiometric approaches are described. The impact of length of acquisition time on the quality of the maps is detailed. These methods may be useful for investigating progression in kidney disease in vivo for rodent models.This chapter is based upon work from the COST Action PARENCHIMA, a community-driven network funded by the European Cooperation in Science and Technology (COST) program of the European Union, which aims to improve the reproducibility and standardization of renal MRI biomarkers. This experimental protocol is complemented by two separate chapters describing the basic concepts and data analysis.
化学交换饱和转移（CEST）被公认为测量 pH 值的主要方法之一，预计该环境变量将成为肾脏疾病的优秀生物标志物。本研究详细描述了使用碘帕醇作为对比剂后，在啮齿动物中监测肾脏 pH 值动态平衡的 CEST MRI 实验方案，包括 pH 值和灌注图的生成。本研究还描述了几种 CEST 技术、采集方案和比率方法。此外，本研究还详细说明了采集时间长短对图谱质量的影响。这些方法可能有助于研究动物模型中肾脏疾病的进展。本文所描述的方法基于 COST 行动 PARENCHIMA 的工作，该行动是一个由社区驱动的网络，由欧盟的欧洲科学与技术合作组织（COST）计划资助，旨在提高肾脏 MRI 生物标志物的可重复性和标准化。本实验方案还辅以另外两章，分别描述了基本概念和数据分析。
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