University of Campania "Luigi Vanvitelli", Napoli, Italy.
Department of Molecular Biotechnology and Health Sciences, University of Torino, Torino, Italy.
Methods Mol Biol. 2021;2216:429-441. doi: 10.1007/978-1-0716-0978-1_25.
Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) can provide a noninvasive way for assessing renal functional information following the administration of a small molecular weight gadolinium-based contrast agent. This method may be useful for investigating renal perfusion and glomerular filtration rates of rodents in vivo under various experimental (patho)physiological conditions. Here we describe a step-by-step protocol for DCE-MRI studies in small animals providing practical notes on acquisition parameters, sequences, T mapping approaches and procedures.This chapters is based upon work from the COST Action PARENCHIMA, a community-driven network funded by the European Cooperation in Science and Technology (COST) program of the European Union, which aims to improve the reproducibility and standardization of renal MRI biomarkers. This experimental protocol chapter is complemented by two separate chapters describing the basic concept and data analysis.
动态对比增强磁共振成像（DCE-MRI）可以为评估小分子量基于钆的造影剂给药后的肾功能信息提供一种非侵入性的方法。该方法可能有助于研究各种实验（病理）生理条件下啮齿动物的肾灌注和肾小球滤过率。在这里，我们描述了小动物 DCE-MRI 研究的分步协议，提供了关于采集参数、序列、T 映射方法和程序的实用说明。这一章基于 COST 行动 PARENCHIMA 的工作，这是一个由社区驱动的网络，由欧盟的欧洲科学技术合作组织（COST）计划资助，旨在提高肾 MRI 生物标志物的重现性和标准化。该实验方案章节由另外两个章节补充，描述了基本概念和数据分析。
