Bacterial Disease Division, Animal and Plant Quarantine Agency, Gyeongsangbuk-do, Republic of Korea.
Bacterial Disease Division, Animal and Plant Quarantine Agency, Gyeongsangbuk-do, Republic of Korea.
J Glob Antimicrob Resist. 2021 Mar;24:443-445. doi: 10.1016/j.jgar.2020.12.022. Epub 2021 Jan 18.
Salmonella enterica Indiana ST17 strain (K16SI097), exclusively found in China, was first isolated in 2016 in Korea from a chicken carcass. This strain contains multidrug-resistant genes, and is particularly resistant to ciprofloxacin (64 μg/mL). In this study, we aimed to elucidate the genomic relationship and compare antimicrobial resistance factors between Korean chicken-derived and Chinese clones of S. Indiana ST17.
The genomic DNA of S. enterica Indiana K16SI097 was sequenced via the combined analysis of 20-kb PacBio SMRTbell library and PacBio RS II. Antimicrobial resistance genes were analysed by the Center for Genomic Epidemiology (http://www.genomicepidemiology.org/). Chromosomal and plasmid DNA of the Korean and Chinese strains were compared.
The K16SI097 genome comprises two contiguous sequences (contigs) amounting to 4 731 335 base pairs with a 51.85% GC content. In total, 4574 protein-coding regions, 84 tRNA genes, and 22 rRNA genes were detected. Among the annotated contigs, 14 antimicrobial resistance genes were detected; DNA gyrase and topoisomerase IV contained mutations. Moreover, chromosomal DNA of K16SI097 and of the published Chinese strain displayed 99.9% similarity. Furthermore, plasmids displayed similar sizes, sequences, and structures.
This is the first report on the complete genome sequence of the high-level ciprofloxacin-resistant S. enterica Indiana ST17 strain isolated in Korea. This genome sequence will help us understand the ST17 strain lineage and its features such as antimicrobial resistance.
沙门氏菌印第安纳 ST17 株（K16SI097）是一种仅在中国发现的菌株，于 2016 年首次在韩国从鸡尸中分离得到。该菌株含有多种耐药基因，对环丙沙星（64μg/mL）的耐药性尤其强。本研究旨在阐明韩国鸡肉来源的和中国分离的 ST17 株之间的基因组关系，并比较其抗菌药物耐药因子。
通过结合使用 20-kb PacBio SMRTbell 文库和 PacBio RS II 对 S. enterica Indiana K16SI097 的基因组 DNA 进行测序。通过基因组流行病学中心（http://www.genomicepidemiology.org/）分析抗菌药物耐药基因。比较韩国和中国分离株的染色体和质粒 DNA。
K16SI097 基因组由两个连续的序列（contigs）组成，总长度为 4731335 个碱基对，GC 含量为 51.85%。共检测到 4574 个蛋白编码区、84 个 tRNA 基因和 22 个 rRNA 基因。在所注释的 contigs 中，检测到 14 个抗菌药物耐药基因；DNA 回旋酶和拓扑异构酶 IV 发生了突变。此外，K16SI097 的染色体 DNA 与已发表的中国分离株显示出 99.9%的相似性。此外，质粒显示出相似的大小、序列和结构。
这是首次报道韩国分离的高水平环丙沙星耐药性沙门氏菌印第安纳 ST17 株的完整基因组序列。该基因组序列将有助于我们了解 ST17 株系及其特征，如抗菌药物耐药性。
