Han Hee-Won, Kwak Jin-Hwan, Jang Tae-Su, Knowles Jonathan Campbell, Kim Hae-Won, Lee Hae-Hyoung, Lee Jung-Hwan
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, Chungcheongnam-do 31116, Korea.
Department of Biomaterials Science, College of Dentistry, Dankook University, Cheonan, Chungcheongnam-do 31116, Korea.
Antibiotics (Basel). 2021 Jan 18;10(1):85. doi: 10.3390/antibiotics10010085.
Multidrug-resistant (MDR) bacteria are increasing due to the abuse and misuse of antibiotics, and nosocomial infections by MDR bacteria are also increasing. The aim of this study was to identify new substances that can target MDR bacteria among 12 plant extracts that are known to have antibacterial effects. The experiments were performed by the disk diffusion test and microdilution minimum inhibitory concentration (MIC) test, as described by the Clinical and Laboratory Standards Institute (CLSI). By screening against methicillin-sensitive (MSSA), grapefruit seed extract (GSE) was selected from 12 plant extracts for subsequent experiments. GSE showed antibacterial effects against methicillin-resistant (MRSA) and vancomycin-resistant (VRSA) in the disk diffusion test. Even at the lowest concentration, GSE showed antibacterial activity in the microdilution MIC test. As a result, we can conclude that GSE is a naturally derived antibacterial substance that exhibits a favorable antibacterial effect even at a very low concentration, so it is a good candidate for a natural substance that can be used to prevent or reduce nosocomial infections as coating for materials used in medical contexts or by mixing a small amount with other materials.
由于抗生素的滥用和误用，多重耐药（MDR）细菌正在增加，MDR细菌引起的医院感染也在增加。本研究的目的是在已知具有抗菌作用的12种植物提取物中鉴定出可靶向MDR细菌的新物质。实验按照临床和实验室标准协会（CLSI）的描述，通过纸片扩散法试验和微量稀释最低抑菌浓度（MIC）试验进行。通过对甲氧西林敏感（MSSA）菌进行筛选，从12种植物提取物中选择了葡萄柚籽提取物（GSE）用于后续实验。在纸片扩散法试验中，GSE对耐甲氧西林（MRSA）和耐万古霉素（VRSA）菌显示出抗菌作用。即使在最低浓度下，GSE在微量稀释MIC试验中也显示出抗菌活性。因此，我们可以得出结论，GSE是一种天然来源的抗菌物质，即使在非常低的浓度下也表现出良好的抗菌效果，因此它是一种很好的天然物质候选物，可作为医疗环境中使用材料的涂层或与其他材料少量混合，用于预防或减少医院感染。
