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氧化应激对[Ru(Phen)]体内和体外实时氧检测的影响。

Influence of Oxidative Stress on Time-Resolved Oxygen Detection by [Ru(Phen)] In Vivo and In Vitro.

机构信息

Center for Interdisciplinary Biosciences, Technology and Innovation Park, P.J. Safarik University in Kosice, Jesenna 5, 041 54 Kosice, Slovakia.

Laboratory for Functional and Metabolic Imaging, Institute of Physics, Swiss Federal Institute of Technology in Lausanne (EPFL), Station 6, Batiment de Chimie, 1015 Lausanne, Switzerland.

出版信息

Molecules. 2021 Jan 18;26(2):485. doi: 10.3390/molecules26020485.

DOI:10.3390/molecules26020485
PMID:33477558
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7831141/
Abstract

Detection of tissue and cell oxygenation is of high importance in fundamental biological and in many medical applications, particularly for monitoring dysfunction in the early stages of cancer. Measurements of the luminescence lifetimes of molecular probes offer a very promising and non-invasive approach to estimate tissue and cell oxygenation in vivo and in vitro. We optimized the evaluation of oxygen detection in vivo by [Ru(Phen)] in the chicken embryo chorioallantoic membrane model. Its luminescence lifetimes measured in the CAM were analyzed through hierarchical clustering. The detection of the tissue oxygenation at the oxidative stress conditions is still challenging. We applied simultaneous time-resolved recording of the mitochondrial probe MitoTracker OrangeCMTMRos fluorescence and [Ru(Phen)] phosphorescence imaging in the intact cell without affecting the sensitivities of these molecular probes. [Ru(Phen)] was demonstrated to be suitable for in vitro detection of oxygen under various stress factors that mimic oxidative stress: other molecular sensors, HO, and curcumin-mediated photodynamic therapy in glioma cancer cells. Low phototoxicities of the molecular probes were finally observed. Our study offers a high potential for the application and generalization of tissue oxygenation as an innovative approach based on the similarities between interdependent biological influences. It is particularly suitable for therapeutic approaches targeting metabolic alterations as well as oxygen, glucose, or lipid deprivation.

摘要

组织和细胞氧合的检测在基础生物学和许多医学应用中都非常重要，特别是在监测癌症早期功能障碍方面。分子探针的发光寿命测量为评估体内和体外组织和细胞氧合提供了一种非常有前途且非侵入性的方法。我们通过鸡胚绒毛尿囊膜模型优化了 [Ru(Phen)] 对体内氧检测的评价。通过层次聚类分析了在 CAM 中测量的其发光寿命。在氧化应激条件下检测组织氧合仍然具有挑战性。我们在不影响这些分子探针灵敏度的情况下，应用同时记录完整细胞中线粒体探针 MitoTracker OrangeCMTMRos 荧光和 [Ru(Phen)] 磷光的时间分辨记录。证明 [Ru(Phen)] 适合在各种模拟氧化应激的应激因素下进行体外氧检测：其他分子传感器、HO 和姜黄素介导的神经胶质瘤癌细胞光动力疗法。最后观察到分子探针的低光毒性。我们的研究为基于相互依赖的生物学影响之间的相似性将组织氧合作为一种创新方法的应用和推广提供了巨大的潜力。它特别适合针对代谢改变以及缺氧、葡萄糖或脂质剥夺的治疗方法。

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3
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