Maiborodin I V, Maslov R V, Ryaguzov M E, Maiborodina V I, Lushnikova E L
Institute of Molecular Pathology and Pathomorphology, Federal Research Center of Fundamental and Translational Medicine, Novosibirsk, Russia.
Center of New Medical Technologies, Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Bull Exp Biol Med. 2022 Nov;174(1):116-124. doi: 10.1007/s10517-022-05659-0. Epub 2022 Nov 28.
The possibility of dissemination of bone marrow multipotent stromal cells stained with Vybrant CM-Dil after injection into an intact and resected liver was studied using luminescence microscopy. Labeled cells were found in the kidneys, spleen, lungs, axillary, mesenteric, and inguinal lymph nodes. We observed dissemination of multipotent stromal cells and their detritus throughout the body that occurred only after filtration in the lungs, where most cells underwent destruction. Perivascularly located macrophages in various organs can phagocytize multipotent stromal cells and their detritus from blood vessels. The content of objects labeled with Vybrant CM-Dil in distant organs was significantly lower after multipotent stromal cell injection into the resected liver, which was associated with the deposition of cells in the damaged area of the organ and their partial entry into the abdominal cavity.
利用发光显微镜研究了注射用Vybrant CM-Dil染色的骨髓多能基质细胞注入完整肝脏和切除肝脏后扩散的可能性。在肾脏、脾脏、肺、腋窝、肠系膜和腹股沟淋巴结中发现了标记细胞。我们观察到多能基质细胞及其碎屑在全身扩散，这种扩散仅在肺部过滤后发生，其中大多数细胞遭到破坏。不同器官中位于血管周围的巨噬细胞可以吞噬来自血管的多能基质细胞及其碎屑。将多能基质细胞注入切除肝脏后，远处器官中用Vybrant CM-Dil标记的物体含量显著降低，这与细胞在器官受损区域的沉积及其部分进入腹腔有关。
