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运动训练或高脂饮食后脂肪来源干细胞分化的脂肪细胞中的代谢组学特征。

Metabolomic Profiles in Adipocytes Differentiated from Adipose-Derived Stem Cells Following Exercise Training or High-Fat Diet.

机构信息

Graduate School of Health and Sports Science, Doshisha University, 1-3 Tatara-Miyakodani, Kyoto 610-0394, Japan.

Organisation for Research Initiatives and Development, Doshisha University, 1-3 Tatara-Miyakodani, Kyoto 610-0394, Japan.

出版信息

Int J Mol Sci. 2021 Jan 19;22(2):966. doi: 10.3390/ijms22020966.

DOI:10.3390/ijms22020966
PMID:33478060
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7835847/
Abstract

Controlling the differentiation potential of adipose-derived stem cells (ADSCs) is attracting attention as a new strategy for the prevention and treatment of obesity. Here, we aimed to observe the effect of exercise training (TR) and high-fat diet (HFD) on the metabolic profiles of ADSCs-derived adipocytes. The rats were divided into four groups: normal diet (ND)-fed control (ND-SED), ND-fed TR (ND-TR), HFD-fed control (HFD-SED), and HFD-fed TR (HFD-TR). After 9 weeks of intervention, ADSCs of epididymal and inguinal adipose tissues were differentiated into adipocytes. In the metabolome analysis of adipocytes after isoproterenol stimulation, 116 metabolites were detected. The principal component analysis demonstrated that ADSCs-derived adipocytes segregated into four clusters in each fat pad. Amino acid accumulation was greater in epididymal ADSCs-derived adipocytes of ND-TR and HFD-TR, but lower in inguinal ADSCs-derived adipocytes of ND-TR, than in the respective controls. HFD accumulated several metabolites including amino acids in inguinal ADSCs-derived adipocytes and more other metabolites in epididymal ones. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that TR mainly affected the pathways related to amino acid metabolism, except in inguinal ADSCs-derived adipocytes of HFD-TR rats. These findings provide a new way to understand the mechanisms underlying possible changes in the differentiation of ADSCs due to TR or HFD.

摘要

控制脂肪来源干细胞（ADSCs）的分化潜能正作为肥胖预防和治疗的新策略受到关注。在这里，我们旨在观察运动训练（TR）和高脂肪饮食（HFD）对 ADSCs 衍生脂肪细胞代谢谱的影响。将大鼠分为四组：正常饮食（ND）喂养对照组（ND-SED）、ND 喂养 TR 组（ND-TR）、HFD 喂养对照组（HFD-SED）和 HFD 喂养 TR 组（HFD-TR）。干预 9 周后，将附睾和腹股沟脂肪组织的 ADSCs 分化为脂肪细胞。在异丙肾上腺素刺激后对脂肪细胞进行代谢组学分析，检测到 116 种代谢物。主成分分析表明，每个脂肪垫中的 ADSCs 衍生脂肪细胞分为四个簇。与各自的对照组相比，ND-TR 和 HFD-TR 大鼠的附睾 ADSCs 衍生脂肪细胞中氨基酸积累更多，而 ND-TR 大鼠的腹股沟 ADSCs 衍生脂肪细胞中氨基酸积累较少。HFD 在腹股沟 ADSCs 衍生脂肪细胞中积累了几种包括氨基酸在内的代谢物，在附睾 ADSCs 衍生脂肪细胞中积累了更多的其他代谢物。京都基因与基因组百科全书富集分析表明，TR 主要影响与氨基酸代谢相关的途径，除了 HFD-TR 大鼠的腹股沟 ADSCs 衍生脂肪细胞。这些发现为理解由于 TR 或 HFD 导致的 ADSCs 分化可能发生的变化的机制提供了新的途径。

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