Murray S, Baillie T A, Davies D S
J Chromatogr. 1977 Nov 1;143(6):541-51. doi: 10.1016/s0378-4347(00)81310-9.
A method is described for the quantitative analysis of (3-methoxy-4-sulphoxyphenyl)-ethylene glycol (MHPG sulphate) in human urine, based on selected ion monitoring gas chromatography--mass spectrometry and using a specifically deuterium-labelled analogue of MHPG sulphate as internal standard. The procedure involves extraction of the urine sample on Amberlite XAD-2, followed by isolation of MHPG sulphate by column chromatography on Sephadex LH-20. Cleavage of the sulphate conjugate and formation of the MHPG tris(trifluoroacetate) derivative are carried out in a one-step reaction, without recourse to enzymic hydrolysis.
本文描述了一种基于选择离子监测气相色谱 - 质谱法，并使用硫酸去甲变肾上腺素（MHPG）硫酸盐的特定氘标记类似物作为内标，对人尿液中（3 - 甲氧基 - 4 - 亚磺酰苯基） - 乙二醇（MHPG硫酸盐）进行定量分析的方法。该程序包括在Amberlite XAD - 2上萃取尿液样品，然后通过Sephadex LH - 20柱色谱法分离MHPG硫酸盐。硫酸盐共轭物的裂解和MHPG三（三氟乙酸酯）衍生物的形成在一步反应中进行，无需酶促水解。
