Peyrin L, Pequignot J M
Psychopharmacology (Berl). 1983;79(1):16-20. doi: 10.1007/BF00433009.
The aim of our study was to investigate the endogenous origin of the three forms of 3-methoxy-4-hydroxyphenylglycol (MHPG) present in human urine and to further examine the hypothesis of an independent (peripheral or central) origin of glucuronide and sulfate conjugates. The urinary levels of free, sulfate, and glucuronide MHPG were determined in control subjects under normal conditions in relation to age, sex, and diet and in two experimental situations known to alter sympathetic activity. The mean daily excretion of total MHPG in a group of 14 men and 14 women was 1780 +/- 122 micrograms, with the free, sulfate, and the glucuronide representing 8% +/- 0.5%, 40% +/- 1.5%, and 52% +/- 1.6%, respectively. No influence of sex, age, or diet was observed on any form. Strong physical activity and anticipatory stress increased norepinephrine excretion and selectively increased MHPG glucuronide levels without changing the free or the sulfate excretion. We conclude that the total amounts of free, sulfate, and glucuronide MHPG found in urine originate from endogenous body pools with no interference of dietary components. The sympathetic nervous system seems to be the main source of glucuronide and arguments are given supporting the central origin of sulfate.
我们研究的目的是调查人尿中三种形式的3-甲氧基-4-羟基苯乙二醇（MHPG）的内源性来源，并进一步检验葡糖醛酸苷和硫酸酯结合物独立（外周或中枢）来源的假说。在正常条件下，测定了对照受试者尿液中游离、硫酸化和葡糖醛酸化MHPG的水平，并将其与年龄、性别和饮食相关联，同时还测定了在已知会改变交感神经活性的两种实验情况下的水平。一组14名男性和14名女性中，总MHPG的平均每日排泄量为1780±122微克，其中游离型、硫酸化型和葡糖醛酸化型分别占8%±0.5%、40%±1.5%和52%±1.6%。未观察到性别、年龄或饮食对任何形式有影响。剧烈体力活动和预期应激会增加去甲肾上腺素排泄，并选择性增加MHPG葡糖醛酸苷水平，而不改变游离型或硫酸化型的排泄。我们得出结论，尿液中游离、硫酸化和葡糖醛酸化MHPG的总量源自体内内源性池，不受饮食成分的干扰。交感神经系统似乎是葡糖醛酸苷的主要来源，并且有证据支持硫酸酯的中枢来源。
