CAS Key Laboratory of Regenerative Biology, South China Institutes for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.
EMBO Rep. 2021 Feb 3;22(2):e51644. doi: 10.15252/embr.202051644. Epub 2021 Jan 22.
Genome architecture and chromatin dynamics govern the fate and identify of a cell. Recent advances in mapping chromatin landscapes offer valuable tools for the acquisition of accurate information regarding chromatin dynamics. Here we discuss recent findings linking chromatin dynamics to cell fate control. Specifically, chromatin undergoes a binary off/on switch during iPSC reprogramming, closing and opening loci occupied by somatic and pluripotency transcription factors, respectively. This logic of a binary off/on switch may also be operational in cell fate control during normal development and implies that further approaches could potentially be developed to direct cell fate changes both in vitro and in vivo.
基因组结构和染色质动力学控制着细胞的命运和身份。近年来，对染色质景观进行绘图的进展为获取有关染色质动力学的准确信息提供了有价值的工具。在这里，我们讨论了将染色质动力学与细胞命运控制联系起来的最新发现。具体来说，在 iPSC 重编程过程中，染色质经历了二元开/关开关，分别关闭和打开由体细胞和多能转录因子占据的基因座。这种二元开/关开关的逻辑在正常发育过程中的细胞命运控制中也可能起作用，这意味着可能会开发出进一步的方法来指导体外和体内的细胞命运变化。
