Applied Entomology and Medical Toxicology Laboratory, Department of Zoology, Government College University, Lahore, Pakistan.
Arch Microbiol. 2021 May;203(4):1281-1292. doi: 10.1007/s00203-020-02128-y. Epub 2021 Jan 22.
Amylase is amongst the most indispensable enzymes that have a large number of applications in laboratories and industries. Mostly, α-amylase is synthesized from microbes such as bacteria, fungi and yeast. Due to the high demand for α-amylase, its synthesis can be enhanced using recombinant DNA technology, different fermentation methods, less expensive and good carbon and nitrogen sources, and optimizing the various parameters during fermentation, e.g., temperature, pH and fermentation duration. Various methods are used to measure the production and activity of synthesized α-amylase like iodine, DNS, NS and dextrinizing methods. The activity of crude α-amylase can be elevated to the maximum level by optimizing the temperature and pH. Some metals also interact with α-amylase and increase its activity like K, Na, Mg and Ca. Some industries such as starch conversion, food, detergent, paper, textile industries and fuel alcohol production extensively utilize α-amylase for their various purposes.
淀粉酶是最不可或缺的酶之一，在实验室和工业中有广泛的应用。大多数情况下，α-淀粉酶是由细菌、真菌和酵母等微生物合成的。由于对α-淀粉酶的需求很高，其合成可以通过重组 DNA 技术、不同的发酵方法、更便宜且良好的碳氮源以及在发酵过程中优化各种参数（例如温度、pH 和发酵时间）来增强。碘、DNS、NS 和糊化方法等各种方法用于测量合成的α-淀粉酶的产量和活性。通过优化温度和 pH，可以将粗α-淀粉酶的活性提高到最高水平。一些金属也与α-淀粉酶相互作用并提高其活性，如 K、Na、Mg 和 Ca。淀粉转化、食品、洗涤剂、造纸、纺织和燃料酒精生产等一些行业广泛利用α-淀粉酶来满足其各种用途。
