Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska Street, 90-236 Lodz, Poland.
Department of Organic and Inorganic Chemistry and Research Chemistry Institute "Andrés M. del Río" (IQAR), University of Alcalá, 28871 Alcalá de Henares, Spain.
J Phys Chem B. 2021 Feb 4;125(4):1196-1206. doi: 10.1021/acs.jpcb.0c10177. Epub 2021 Jan 22.
The use of nonviral carriers based on nanomaterials is a promising strategy for modern gene therapy aimed at protecting the genetic material against degradation and enabling its efficient cellular uptake. To improve the effectiveness of nanocarriers , they are often modified with poly(ethylene glycol) (PEG) to reduce their toxicity, limit nonspecific binding by proteins in the bloodstream, and extend blood half-life. Thus, the selection of an appropriate degree of surface PEGylation is crucial to preserve the interaction of nanoparticles with the genetic material and to ensure its efficient transport to the site of action. Our research focuses on the use of innovative gold nanoparticles (AuNPs) coated with cationic carbosilane dendrons as carriers of siRNA. In this study, using dynamic light scattering and zeta potential measurements, circular dichroism, and gel electrophoresis, we investigated dendronized AuNPs modified to varying degrees with PEG in terms of their interactions with siRNA and thrombin to select the most promising PEGylated carrier for further research.
基于纳米材料的非病毒载体是现代基因治疗的一种有前途的策略，旨在保护遗传物质免受降解，并使其能够有效地被细胞摄取。为了提高纳米载体的有效性，通常用聚乙二醇（PEG）对其进行修饰，以降低其毒性，限制血液中蛋白质的非特异性结合，并延长血液半衰期。因此，选择适当的表面 PEG 化程度对于保持纳米颗粒与遗传物质的相互作用以及确保其有效运输到作用部位至关重要。我们的研究重点是使用涂有阳离子碳硅烷树枝状大分子的新型金纳米颗粒（AuNPs）作为 siRNA 的载体。在这项研究中，我们使用动态光散射和zeta 电位测量、圆二色性和凝胶电泳，研究了不同程度修饰 PEG 的树枝化 AuNPs 与 siRNA 和凝血酶的相互作用，以选择最有前途的 PEG 化载体进行进一步研究。
