Dpto. de Química Orgánica y Química Inorgánica, Universidad de Alcalá, Campus Universitario, E-28871 Alcalá de Henares, Madrid, Spain.
Dalton Trans. 2017 Jul 11;46(27):8736-8745. doi: 10.1039/c6dt03791g.
Gold nanoparticles (AuNPs) and polycationic macromolecules are used as gene carriers. Their behaviour is dependent on several factors, such as the size and type of the framework, charge, etc. We have combined both types of systems and prepared AuNPs covered with cationic carbosilane dendrons with the aim to evaluate their biocompatibility. Water soluble dendronized cationic AuNPs were prepared following a straightforward procedure from dendrons, a gold precursor and a reducing agent in water and were characterized by H NMR, transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analysis (TGA), ultraviolet spectroscopy (UV), and zeta potential (ZP). The biological properties of dendrons and AuNPs were determined by hemolysis, platelet aggregation and lymphocyte proliferation. These assays reflect modification of dendron properties when covering nanoparticles. For dendrons, hemolysis and platelet aggregation are generation dependent whilst, for AuNPs these properties are related to the bigger size of NPs. On the other hand, none of the systems induced lymphocyte proliferation. Selected cationic dendrons and AuNPs were chosen for gene delivery experiments employing a small interference RNA (siRNA Nef) against human immunodeficiency virus (HIV).
金纳米粒子（AuNPs）和聚阳离子高分子被用作基因载体。它们的行为取决于几个因素，如骨架的大小和类型、电荷等。我们将这两种系统结合起来，制备了带有阳离子碳硅烷树枝状大分子的 AuNPs，旨在评估它们的生物相容性。水溶性树枝状阳离子 AuNPs 是按照一种简单的方法，从树枝状大分子、金前体和还原剂在水中制备的，并通过氢核磁共振（1H NMR）、透射电子显微镜（TEM）、动态光散射（DLS）、热重分析（TGA）、紫外光谱（UV）和 Zeta 电位（ZP）进行了表征。树枝状大分子和 AuNPs 的生物学性质通过溶血、血小板聚集和淋巴细胞增殖来确定。这些测定反映了纳米颗粒覆盖时树枝状大分子性质的修饰。对于树枝状大分子，溶血和血小板聚集与代际有关，而对于 AuNPs，这些性质与 NPs 的较大尺寸有关。另一方面，没有任何一种系统诱导淋巴细胞增殖。选择了一些阳离子树枝状大分子和 AuNPs 进行基因传递实验，使用了针对人类免疫缺陷病毒（HIV）的小干扰 RNA（siRNA Nef）。
