Dpto. de Química Orgánica y Química Inorgánica, Universidad de Alcalá (UAH), Campus Universitario, E-28871 Alcalá de Henares (Madrid), Spain; Instituto de Investigación Química "Andrés M. del Río" (IQAR), Universidad de Alcalá (UAH), Spain; Networking Research Center for Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain; Instituto Ramón y Cajal de Investigación Sanitaria, IRYCIS, Spain.
Dpto. de Química Orgánica y Química Inorgánica, Universidad de Alcalá (UAH), Campus Universitario, E-28871 Alcalá de Henares (Madrid), Spain; Instituto de Investigación Química "Andrés M. del Río" (IQAR), Universidad de Alcalá (UAH), Spain; Networking Research Center for Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain; Instituto Ramón y Cajal de Investigación Sanitaria, IRYCIS, Spain.
Int J Pharm. 2019 Oct 5;569:118591. doi: 10.1016/j.ijpharm.2019.118591. Epub 2019 Aug 5.
This work focuses on preparation of silver nanoparticles (AgNP) covered with cationic carbosilane dendrons and poly(ethylene glycol) (PEG). It is well known that AgNP and cationic carbosilane dendritic systems present antibacterial properties. On the other hand, PEG ligand provides antifouling properties and improved biocompatibility. Hence, combination of both ligands, carbosilane dendrons and PEG, on the AgNP surface can be a way to improve antibacterial capacity of AgNP. The new family of heterofunctionalized AgNP has been directly synthesized using silver precursor and cationic carbosilane dendrons and PEG ligands containing a thiol moiety. AgNP were characterized by TEM, TGA, UV, H NMR, DLS, Z potential, XRD. The antibacterial capacity of these systems was evaluated against E. coli and S. aureus. The results confirmed the influence of both silver core and cationic carbosilane dendrons on the activity of these systems. The behaviour obtained for PEGylated systems were slightly lower than for non-PEGylated AgNP. However, hemolysis assays demonstrated that this decrease was compensated for by the greater biocompatibility. To more completely characterize the improvements of PEGylation on dendronized AgNP, one non-PEGylated and one PEGylated AgNP were tested for resistance in a planktonic state. Both AgNPs barely affected the minimum inhibitory concentration (MIC) whereas reference antibiotics generated significant resistance. In addition, relevant improvement in biofilm inhibition was achieved by dendronized AgNP after PEGylation.
这项工作专注于制备被阳离子碳硅烷树枝状大分子和聚（乙二醇）（PEG）覆盖的银纳米粒子（AgNP）。众所周知，AgNP 和阳离子碳硅烷树枝状大分子系统具有抗菌性能。另一方面，PEG 配体提供抗污性能和提高的生物相容性。因此，将两种配体，即碳硅烷树枝状大分子和 PEG，结合在 AgNP 表面上，可以提高 AgNP 的抗菌能力。新的杂官能化 AgNP 家族已使用银前体和含有巯基部分的阳离子碳硅烷树枝状大分子和 PEG 配体直接合成。通过 TEM、TGA、UV、H NMR、DLS、Z 电位、XRD 对 AgNP 进行了表征。评估了这些系统对大肠杆菌和金黄色葡萄球菌的抗菌能力。结果证实了银核和阳离子碳硅烷树枝状大分子对这些系统活性的影响。PEG 化系统的行为略低于非 PEG 化 AgNP。然而，溶血试验表明，这种降低被更大的生物相容性所补偿。为了更全面地描述 PEG 化对树枝化 AgNP 的改进，测试了一种非 PEG 化和一种 PEG 化的 AgNP 对浮游状态下的耐药性。两种 AgNP 几乎都没有影响最小抑菌浓度（MIC），而参考抗生素则产生了显著的耐药性。此外，PEG 化后的树枝化 AgNP 在抑制生物膜方面也有显著的改善。
