State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China; Chuxiong Medical College, Chuxiong, 675005, China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Carbohydr Polym. 2021 Mar 15;256:117513. doi: 10.1016/j.carbpol.2020.117513. Epub 2020 Dec 15.
Marine sulfated polysaccharides have aroused widespread concern for their various structures and bioactivities. Peroxide depolymerization is a common strategy in analysis of structures and structure-activity relationships of polysaccharides. However, confirming the depolymerization process and exact structures of the degradation products is still a considerable challenge. This study reported the structures of a fucan sulfate (FS) from sea cucumber Stichopus herrmanni and its depolymerized products (dFS) prepared by peroxide degradation. The FS was elucidated with a highly regular structure, {-3)-L-Fuc-(α1-}. Structure analysis of oligosaccharides purified from dFS suggested that peroxide degradation involved in cleavage of glycosidic bonds and oxidative modification of reducing end of sugar residue, while no break in sugar ring was observed. Both FS and series of dFSs exhibited significant anticoagulant activities due to their anti-thrombin effects in presence of heparin cofactor II and their potencies were related to their molecular sizes, dFS with ∼ 20 kDa showed the strongest activity.
海洋硫酸多糖因其多种结构和生物活性而引起广泛关注。过氧化物降解是分析多糖结构和结构-活性关系的常用策略。然而，确认降解过程和降解产物的确切结构仍然是一个相当大的挑战。本研究报道了海参硫酸岩藻聚糖（FS）及其过氧化物降解产物（dFS）的结构。FS 具有高度规则的结构，{-3)-L-Fuc-(α1-}。从 dFS 中分离得到的寡糖的结构分析表明，过氧化物降解涉及糖苷键的断裂和糖残基还原端的氧化修饰，而没有观察到糖环的断裂。FS 和一系列 dFS 都由于在肝素辅因子 II 存在下的抗凝血酶作用而表现出显著的抗凝活性，其活性与其分子量有关，约 20 kDa 的 dFS 表现出最强的活性。
