Key Laboratory of Marine Drugs, Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China.
Key Laboratory of Marine Drugs, Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China; Marine Biomedical Research Institute of Qingdao, Qingdao, 266071, China.
Carbohydr Polym. 2021 Mar 15;256:117529. doi: 10.1016/j.carbpol.2020.117529. Epub 2020 Dec 17.
Fucoidans from brown seaweed shows various bioactive properties and promising prospects in biomedical field. Here, a novel fucoidan (F-4) was extracted and purified from Durvillaea antarctica. The structure of F-4 was characterized by HPLC, HPGPC, GC-MS, together with IR and NMR spectral analysis. F-4 is a sulfated polysaccharide mainly composed of fucose (Fuc), galactose (Gal), and glucose (Glc) in a molar ratio of 26.4: 7.1: 1.0. The backbone of F-4 is composed of (1→3) and (1→4)-linked-α-L-Fucp residues, which sulfated at C-4 or C-2 positions and branched with α-L-Fuc, β-D-Gal, and β-D-Glc residues. Furthermore, F-4 can effectively promote the growth of leukocyte in a mouse model induced by cyclophosphamide, possibly by activating hematopoietic progenitor cells and regulating the hematopoietic microenvironment of bone marrow. Our data provide useful information for further investigation of fucoidan in the treatment of chemotherapy-induced leukopenia.
褐藻来源的岩藻聚糖具有多种生物活性，在生物医学领域具有广阔的应用前景。本研究从南极羽藻中提取并纯化得到一种新型岩藻聚糖（F-4）。采用 HPLC、HPGPC、GC-MS 联用以及 IR、NMR 光谱分析等方法对 F-4 的结构进行了表征。结果表明，F-4 是一种主要由岩藻糖（Fuc）、半乳糖（Gal）和葡萄糖（Glc）组成的硫酸多糖，摩尔比为 26.4:7.1:1.0。F-4 的主链由（1→3）和（1→4）-连接的α-L-Fucp 残基组成，在 C-4 或 C-2 位硫酸化，并带有α-L-Fuc、β-D-Gal 和β-D-Glc 支链。此外，F-4 可有效促进环磷酰胺诱导的白细胞生成，可能通过激活造血祖细胞和调节骨髓造血微环境来发挥作用。本研究为进一步研究岩藻聚糖在治疗化疗引起的白细胞减少症中的作用提供了有价值的信息。
