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海洋浮霉菌门中顽固岩藻聚糖的分解机制

Mechanisms of recalcitrant fucoidan breakdown in marine Planctomycetota.

作者信息

Pérez-Cruz Carla, Moraleda-Montoya Alicia, Liébana Raquel, Terrones Oihana, Arrizabalaga Uxue, García-Alija Mikel, Lorizate Maier, Martínez Gascueña Ana, García-Álvarez Isabel, Nieto-Garai Jon Ander, Olazar-Intxausti June, Rodríguez-Colinas Bárbara, Mann Enrique, Chiara José Luis, Contreras Francesc-Xabier, Guerin Marcelo E, Trastoy Beatriz, Alonso-Sáez Laura

机构信息

AZTI, Marine Research, Basque Research and Technology Alliance (BRTA), Sukarrieta, Spain.

Structural Glycoimmunology Laboratory, Biobizkaia Health Research Institute, Barakaldo, Spain.

出版信息

Nat Commun. 2024 Dec 30;15(1):10906. doi: 10.1038/s41467-024-55268-w.

DOI:10.1038/s41467-024-55268-w
PMID:39738071
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11685898/
Abstract

Marine brown algae produce the highly recalcitrant polysaccharide fucoidan, contributing to long-term oceanic carbon storage and climate regulation. Fucoidan is degraded by specialized heterotrophic bacteria, which promote ecosystem function and global carbon turnover using largely uncharacterized mechanisms. Here, we isolate and study two Planctomycetota strains from the microbiome associated with the alga Fucus spiralis, which grow efficiently on chemically diverse fucoidans. One of the strains appears to internalize the polymer, while the other strain degrades it extracellularly. Multi-omic approaches show that fucoidan breakdown is mediated by the expression of divergent polysaccharide utilization loci, and endo-fucanases of family GH168 are strongly upregulated during fucoidan digestion. Enzymatic assays and structural biology studies reveal how GH168 endo-fucanases degrade various fucoidan cores from brown algae, assisted by auxiliary hydrolytic enzymes. Overall, our results provide insights into fucoidan processing mechanisms in macroalgal-associated bacteria.

摘要

海洋褐藻产生高度难降解的多糖岩藻依聚糖，这有助于长期的海洋碳储存和气候调节。岩藻依聚糖被专门的异养细菌降解，这些细菌利用 largely 未被表征的机制促进生态系统功能和全球碳周转。在这里，我们从与螺旋藻相关的微生物群中分离并研究了两种浮霉菌门菌株，它们能在化学性质多样的岩藻依聚糖上高效生长。其中一种菌株似乎将聚合物内化，而另一种菌株则在细胞外降解它。多组学方法表明，岩藻依聚糖的分解是由不同的多糖利用位点的表达介导的，并且在岩藻依聚糖消化过程中，GH168 家族的内切岩藻聚糖酶被强烈上调。酶学分析和结构生物学研究揭示了 GH168 内切岩藻聚糖酶如何在辅助水解酶的协助下，降解褐藻中的各种岩藻依聚糖核心。总体而言，我们的结果为大型藻类相关细菌中的岩藻依聚糖加工机制提供了见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/fd7adf312512/41467_2024_55268_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/9afd03076a45/41467_2024_55268_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/0e98ec013bc4/41467_2024_55268_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/d4541808ef12/41467_2024_55268_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/9a38e603fe6f/41467_2024_55268_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/a8d828866671/41467_2024_55268_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/42538d5c1455/41467_2024_55268_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/8c02098759a1/41467_2024_55268_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/2a866034b293/41467_2024_55268_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/fd7adf312512/41467_2024_55268_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/9afd03076a45/41467_2024_55268_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/0e98ec013bc4/41467_2024_55268_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/d4541808ef12/41467_2024_55268_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/9a38e603fe6f/41467_2024_55268_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/a8d828866671/41467_2024_55268_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/42538d5c1455/41467_2024_55268_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/8c02098759a1/41467_2024_55268_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/2a866034b293/41467_2024_55268_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/244b/11685898/fd7adf312512/41467_2024_55268_Fig9_HTML.jpg

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