发光弧菌：实验室培养、储存和常见表型分析。
Vibrio fischeri: Laboratory Cultivation, Storage, and Common Phenotypic Assays.
机构信息
Department of Microbiology and Immunology, Loyola University Chicago, Maywood, Illinois.
出版信息
Curr Protoc Microbiol. 2020 Jun;57(1):e103. doi: 10.1002/cpmc.103.
Vibrio fischeri is a nonpathogenic organism related to pathogenic Vibrio species that can be readily grown and stored with common laboratory equipment. In this article, protocols for routine growth, storage, and phenotypic assessment of V. fischeri, as well as recipes for useful media, are included. Specifically, this article describes procedures and considerations for growth of this microbe in complex and minimal media. It also describes assays for biofilm formation, motility, and bioluminescence, three commonly assessed phenotypes of V. fischeri. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Growth of V. fischeri from frozen stocks Basic Protocol 2: Growth of V. fischeri in rich, undefined liquid medium Alternate Protocol 1: Growth of V. fischeri in minimal medium Basic Protocol 3: Storage of V. fischeri in frozen stocks Basic Protocol 4: Biofilm assay on solid agar Alternate Protocol 2: Biofilm assay in shaking liquid culture Alternate Protocol 3: Biofilm assay in static liquid culture Basic Protocol 5: Motility assay Basic Protocol 6: Luminescence assay.
发光杆菌是一种与致病性弧菌属相关的非致病性生物体，它可以用常见的实验室设备来方便地生长和储存。本文介绍了发光杆菌的常规生长、储存和表型评估的方案，以及有用的培养基配方。具体而言，本文描述了在复杂和基础培养基中培养这种微生物的程序和注意事项。它还描述了生物膜形成、运动性和生物发光三种常见发光杆菌表型的测定方法。© 2020 威利父子公司。基础方案 1：从冷冻库存中生长发光杆菌基础方案 2：在丰富、未定义的液体培养基中生长发光杆菌备选方案 1：在基础培养基中生长发光杆菌基础方案 3：在冷冻库存中储存发光杆菌基础方案 4：固体琼脂上的生物膜测定备选方案 2：在摇瓶液体培养物中的生物膜测定备选方案 3：在静态液体培养物中的生物膜测定基础方案 5：运动性测定基础方案 6：发光测定。
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