Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of Life Science and Engineering, Northwest Minzu University, Lanzhou, Gansu, China; Science Experimental Center, Northwest Minzu University, Lanzhou, Gansu, China.
Gene. 2021 Apr 5;775:145444. doi: 10.1016/j.gene.2021.145444. Epub 2021 Jan 21.
In a previous study, the cyclic AMP response element-binding protein 1 (CREB1) gene, which is likely involved in the regulation of fat metabolism in sheep adipose tissue, was identified using RNA sequencing. CREB1 is a transcription factor that participates in the regulation of cell proliferation, differentiation, and survival as well as energy metabolism. Therefore, based on preliminary studies, this study aimed to reveal the correlation between the insertion/deletion (indel) polymorphism of the CREB1 gene and sheep growth traits. One insertion variation of the ovine CREB1 gene, C3-ins-26 bp, was investigated in 1847 Chinese and Mongolian sheep breeds. The minor allele frequencies in the CREB1 gene varied from 0.021 to 0.938. Further, statistical analyses indicated that the C3-ins-26 bp indel in the CREB1 gene was significantly related to various body measurements (body length, height, and index; chest width, depth, and width index; cannon circumference index; and height at the hip cross) in a Tan sheep population (p < 0.05). Collectively, these findings may provide important insights into marker-assisted selection of sheep.
在之前的一项研究中，使用 RNA 测序鉴定了可能参与绵羊脂肪组织脂肪代谢调节的环磷酸腺苷反应元件结合蛋白 1 (CREB1) 基因。CREB1 是一种转录因子，参与细胞增殖、分化和存活以及能量代谢的调节。因此，基于初步研究，本研究旨在揭示 CREB1 基因插入/缺失（indel）多态性与绵羊生长性状之间的相关性。在 1847 只中国和蒙古绵羊品种中研究了绵羊 CREB1 基因的一个插入变异，C3-ins-26bp。在 CREB1 基因中，次要等位基因频率从 0.021 到 0.938 不等。此外，统计分析表明，在滩羊群体中，CREB1 基因的 C3-ins-26bp 插入缺失与各种体尺（体长、体高和指数；胸宽、胸深和胸宽指数；管围指数；坐骨十字高度）显著相关（p<0.05）。总之，这些发现可能为绵羊的标记辅助选择提供重要的见解。
