State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha 410081, Hunan, PR China.
State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education Ministry, Hunan Normal University, Changsha 410081, Hunan, PR China.
Genomics. 2021 Mar;113(2):595-605. doi: 10.1016/j.ygeno.2021.01.004. Epub 2021 Jan 21.
Triploid crucian carp (TCC) is obtained by hybridization of female diploid red crucian carp (Carassius auratus red var., RCC) and male allotetraploid hybrids. In this study, high-throughput sequencing was used to conduct the transcriptome analysis of the female hypothalamus of diploid RCC, diploid common carp (Cyprinus carpio L., CC) and TCC. The key functional expression genes of the hypothalamus were obtained through functional gene annotation and differential gene expression screening. A total of 71.56 G data and 47,572 genes were obtained through sequencing and genome mapping, respectively. The Fuzzy Analysis Clustering assigned the differentially expressed genes (DEGs) into eight groups, two of which, overdominance expression (6005, 12.62%) and underdominance expression (3849, 8.09%) in TCC were further studied. KEGG enrichment analysis showed that the DEGs in overdominance were mainly enriched in four pathways. The expression of several fertility-related genes was lower levels in TCC, whereas the expression of several growth-related genes and immune-related genes was higher levels in TCC. Besides, 15 DEGs were verified by quantitative real-time PCR (qPCR). The present study can provide a reference for breeding sterility, fast-growth, and disease-resistant varieties by distant hybridization.
三倍体鲫鱼（TCC）是通过雌性二倍体红鲫鱼（Carassius auratus red var.，RCC）和雄性异源四倍体杂种的杂交获得的。本研究采用高通量测序技术对二倍体 RCC、二倍体鲤鱼（Cyprinus carpio L.，CC）和 TCC 的雌性下丘脑进行转录组分析。通过功能基因注释和差异基因表达筛选，获得下丘脑的关键功能表达基因。通过测序和基因组映射，分别获得了 71.56 G 数据和 47,572 个基因。模糊分析聚类将差异表达基因（DEGs）分为八组，其中两组，即 TCC 中的超显性表达（6005，12.62%）和亚显性表达（3849，8.09%）进一步研究。KEGG 富集分析表明，超显性表达的 DEGs 主要富集在四条途径中。TCC 中的几种与生育能力相关的基因表达水平较低，而几种与生长和免疫相关的基因表达水平较高。此外，通过定量实时 PCR（qPCR）验证了 15 个 DEGs。本研究可为远缘杂交培育不育、快速生长和抗病品种提供参考。
