Botany & Microbiology Department, Faculty of Science, Zagazig University, Zagazig, Egypt.
Department of Biology, College of Science, University of Hafr Al Batin, P.O. Box 1803, Hafr Al Batin, Saudi Arabia.
Bioprocess Biosyst Eng. 2021 Apr;44(4):841-854. doi: 10.1007/s00449-020-02494-3. Epub 2021 Jan 24.
The progress of nanoparticles production by eco-friendly route, with desirable chemical and physical characteristics, and their application in helpful fields is still under investigation. Therefore, this study aimed at biosynthesis, characterization, and biomedical applications of silver nanoparticles (AgNPs) using yeasts metabolite. The yeast strains, Pichia kudriavzeviiHA-NY2 and Saccharomyces uvarumHA-NY3, were used for extracellular biosynthesis of AgNPsK and AgNPsU, respectively. AgNPs were characterized by UV-visible spectrophotometry, transmission electron microscopy (TEM), Fourier Transformed Infrared (FTIR) spectrum and dynamic light scatter (DLS). TEM image showed well dispersed round and cubic regular particles with size ranges of 12.4 ± 6.02 nm for AgNPsU and 20.655 ± 9.48 nm for AgNPsK. According to DLS analysis, the mean size diameters of AgNPsU and AgNPsK were 20.3-21.5 and 29.6-30.14 nm, respectively. AgNPs showed highly significant inhibitory activity against gram-positive bacteria (Bacillus subtilis ATCC6633 and Staphylococcus aureus ATCC29213), gram-negative bacteria (Pseudomonas aeruginosa ATCC27953), Candida tropicalis ATCC750, and Fusarium oxysporium NRC21. The anti-inflammatory activity of AgNPs revealed that paw edema was inhibited by the oral administration of the two biosynthesized silver-nanoparticles. In addition, they showed carrageenan activity nearest to indomethacin. All fabricated AgNPs showed a significant analgesic effect after one hour of administration, which was comparable to aspirin. Further, both AgNPsK and AgNPsU demonstrated a significant anticancer activity against HCT-116 (Colon cell line) with IC50 values 0.29, 0.24 µg ml, respectively, and PC3 (Prostate cell line) with IC50 values 0.57, 0.50 µg ml, respectively. No ulcerogenic effects of AgNPs were detected on the rats' stomach and it was safe on the gastric profile.
通过环保途径生产具有理想化学和物理特性的纳米粒子的进展，以及它们在有益领域的应用仍在研究中。因此，本研究旨在利用酵母代谢物生物合成、表征和生物医学应用银纳米粒子（AgNPs）。使用毕赤酵母 Pichia kudriavzeviiHA-NY2 和酿酒酵母 Saccharomyces uvarumHA-NY3 分别进行胞外合成 AgNPsK 和 AgNPsU。AgNPs 采用紫外-可见分光光度法、透射电子显微镜（TEM）、傅里叶变换红外（FTIR）光谱和动态光散射（DLS）进行表征。TEM 图像显示，AgNPsU 的尺寸范围为 12.4±6.02nm，AgNPsK 的尺寸范围为 20.655±9.48nm，均为良好分散的圆形和立方规则颗粒。根据 DLS 分析，AgNPsU 和 AgNPsK 的平均粒径分别为 20.3-21.5nm 和 29.6-30.14nm。AgNPs 对革兰氏阳性菌（枯草芽孢杆菌 ATCC6633 和金黄色葡萄球菌 ATCC29213）、革兰氏阴性菌（铜绿假单胞菌 ATCC27953）、热带假丝酵母 ATCC750 和尖孢镰刀菌 NRC21 具有高度的抑制活性。AgNPs 的抗炎活性表明，两种生物合成的银纳米粒子经口服给药可抑制爪肿胀。此外，它们对卡拉胶的活性与吲哚美辛最接近。给药 1 小时后，所有合成的 AgNPs 均表现出显著的镇痛作用，与阿司匹林相当。此外，AgNPsK 和 AgNPsU 对 HCT-116（结肠细胞系）的 IC50 值分别为 0.29 和 0.24μg/ml，对 PC3（前列腺细胞系）的 IC50 值分别为 0.57 和 0.50μg/ml，均表现出显著的抗癌活性。AgNPs 对大鼠胃无溃疡形成作用，对胃剖面安全。
