Institute for Laboratory Animal Research, Section of Life and Function Materials, Science Research Center, Kochi University, Nankoku, Kochi, Japan.
Department of Parasitology, Kochi Medical School, Kochi University, Nankoku, Kochi, Japan.
Methods Mol Biol. 2021;2241:133-137. doi: 10.1007/978-1-0716-1095-4_11.
Eosinophils are differentiated in the bone marrow and transit through the blood circulation to home into tissues primarily under the regulation of IL-5. Because the number of eosinophils in the peripheral blood is relatively low under normal conditions, in vivo functional studies of eosinophils remain extremely difficult. Increasing their numbers in vivo might be useful for assessing eosinophil activities during parasite infections, allergic inflammation, and so on. Here, we provide a method for eosinophil expansion using IL-5 gene transfer by electroporation in vivo.
嗜酸性粒细胞在骨髓中分化，并通过血液循环迁移到组织中，主要受白细胞介素 5（IL-5）的调节。由于正常情况下外周血中的嗜酸性粒细胞数量相对较低，因此体内嗜酸性粒细胞的功能研究仍然极其困难。增加体内嗜酸性粒细胞的数量可能有助于评估寄生虫感染、过敏炎症等期间嗜酸性粒细胞的活性。在这里，我们提供了一种通过体内电穿孔转染白细胞介素 5（IL-5）基因来扩增嗜酸性粒细胞的方法。
