Department of Oncology, Jiangxi Maternal and Child Health Hospital , Nanchang, Jiangxi, China.
Department of Pathology, Jiangxi Maternal and Child Health Hospital , Nanchang, Jiangxi, China.
Bioengineered. 2021 Dec;12(1):440-449. doi: 10.1080/21655979.2021.1871817.
Previous studies reported that miR-330-3p was involved in the progression of several cancers, but the potential roles of miR-330-3p in ovarian cancer (OC) were unclear. In the current study, we aimed to explore the expression pattern and functions of miR-330-3p in OC. The expression level of miR-330-3p in OC tissues and cell lines was detected using RT-qPCR. The proliferation, migration and invasion of OC cells were detected using CCK-8 assay and transwell assay, respectively. Bioinformatics analysis and luciferase reporter assay were used to analyze the targeted binding site of miR-330-3p and RIPK4. The results showed that miR-330-3p was significantly downregulated in OC tissues and cell lines. Overexpression of miR-330-3p inhibited the proliferation, migration and invasion of OC cells. Mechanistically, a dual-luciferase reported assay showed that RIPK4 is a target gene of miR-330-3p. Furthermore, rescue experiments revealed that miR-330-3p suppressed the proliferation, migration and invasion of OC cells by targeting RIPK4. In summary, our findings indicated that miR-330-3p suppressed the progression of OC by targeting RIPK4. Our results indicated that miR-330-3p/RIPK4 axis might act as a novel therapeutic target for OC treatment.
先前的研究报告表明，miR-330-3p 参与了几种癌症的进展，但 miR-330-3p 在卵巢癌（OC）中的潜在作用尚不清楚。在本研究中，我们旨在探讨 miR-330-3p 在 OC 中的表达模式和功能。使用 RT-qPCR 检测 OC 组织和细胞系中 miR-330-3p 的表达水平。使用 CCK-8 测定法和 Transwell 测定法分别检测 OC 细胞的增殖、迁移和侵袭。生物信息学分析和荧光素酶报告基因测定用于分析 miR-330-3p 和 RIPK4 的靶向结合位点。结果表明，miR-330-3p 在 OC 组织和细胞系中显著下调。miR-330-3p 的过表达抑制了 OC 细胞的增殖、迁移和侵袭。机制上，双荧光素酶报告基因测定显示 RIPK4 是 miR-330-3p 的靶基因。此外，挽救实验表明，miR-330-3p 通过靶向 RIPK4 抑制 OC 细胞的增殖、迁移和侵袭。总之，我们的研究结果表明，miR-330-3p 通过靶向 RIPK4 抑制 OC 的进展。我们的结果表明，miR-330-3p/RIPK4 轴可能成为 OC 治疗的新的治疗靶点。
