El-Shehawi Ahmed M, Alotaibi Saqer S, Elseehy Mona M
Department of Biotechnology, College of Science, Taif University, P.O. Box 11099 21944 Taif, Saudi Arabia.
Department of Genetics, Faculty of Agriculture, University of Alexandria, 21527 Alexandria, Egypt.
Cytol Genet. 2020;54(6):588-604. doi: 10.3103/S0095452720060031. Epub 2021 Jan 15.
The COVID-19 corona virus has become a world pandemic which started in December 2019 in Wuhan, China with no confirmed biological source. Various countries reported the genomic sequence of different isolates obtained from infected patients. This allowed us to obtain a number of 38 isolates of full genomic sequences. Alignment of nucleotide (nt) sequence was carried out using Clustal Omega multiple alignment service at the EBI website. Alignment of nt sequence and phylogenetic relationship revealed that the COVID-19 is a new viral strain and its biological source has not been yet detected. The expected orf pattern was different among isolates obtained from the same country or different countries as well as from SARS-CoV isolates or bats CoV suggesting different virus human interaction possibilities during infection and severity. All isolates had the main five orfs (1ab, S, M, N, E), whereas they differed in the expected accessory orfs. Being with the biological source of COVID-19 undetected, the role of human endogenous retrovirus (HERVs) in the regulation of the host cell gene expression or the encoding for products that could modulate COVID-19 infection and the spectrum of its symptoms is discussed.
2019年12月在中国武汉开始出现的新型冠状病毒肺炎冠状病毒已成为全球大流行疾病，其生物来源尚未得到证实。各国报告了从感染患者身上分离出的不同毒株的基因组序列。这使我们获得了38个完整基因组序列的毒株。使用欧洲生物信息研究所（EBI）网站上的Clustal Omega多重比对服务对核苷酸（nt）序列进行了比对。核苷酸序列比对和系统发育关系表明，新型冠状病毒肺炎是一种新的病毒株，其生物来源尚未被发现。从同一国家或不同国家获得的毒株以及与严重急性呼吸综合征冠状病毒（SARS-CoV）毒株或蝙蝠冠状病毒毒株相比，预期的开放阅读框（orf）模式不同，这表明在感染和严重程度方面存在不同的病毒与人类相互作用可能性。所有毒株都有主要的五个开放阅读框（1ab、S、M、N、E），而它们在预期的辅助开放阅读框方面有所不同。鉴于新型冠状病毒肺炎的生物来源尚未被发现，本文讨论了人类内源性逆转录病毒（HERVs）在调节宿主细胞基因表达或编码可能调节新型冠状病毒肺炎感染及其症状谱的产物方面的作用。
