Jacquemond M, Lauquin G J
Station de Pathologie Végétale, INRA, Montfavet, France.
Biochem Biophys Res Commun. 1988 Feb 29;151(1):388-95. doi: 10.1016/0006-291x(88)90605-5.
Two isolates of cucumber mosaic virus (CMV)-associated satellite RNA, differing in their biological properties, have been reverse transcribed. One was able to induce the tomato necrotic syndrome whereas the other one attenuated fernleaf symptoms on tomato plants after co-inoculation with the helper virus. cDNAs representing partial or full-length copies have been cloned in the plasmid pAT 153 and sequenced. The two RNAs showed a very limited number of variations (2 to 5 substitutions depending on the clones and a one base deletion). Full-length cDNA copies possessed the same biological properties that characterized the parent satellite RNA. Efficiency of the cDNA depended upon its form in the inoculum (circular or linear plasmid or excised cDNA) and upon the form of the helper virus (viral RNAs or virions) with which it seemed to compete for installation and/or expression.
已对两种生物学特性不同的黄瓜花叶病毒（CMV）相关卫星RNA分离株进行了逆转录。其中一种能够诱发番茄坏死综合征，而另一种在与辅助病毒共同接种后可减轻番茄植株的蕨叶症状。代表部分或全长拷贝的cDNA已克隆到质粒pAT 153中并进行了测序。这两种RNA显示出非常有限的变异数量（根据克隆情况有2至5个替换以及一个碱基缺失）。全长cDNA拷贝具有与亲本卫星RNA相同的生物学特性。cDNA的效率取决于其在接种物中的形式（环状或线性质粒或切除的cDNA）以及辅助病毒的形式（病毒RNA或病毒粒子），它似乎与辅助病毒竞争安装和/或表达。
