Collmer C W, Kaper J M
Biochem Biophys Res Commun. 1986 Feb 26;135(1):290-6. doi: 10.1016/0006-291x(86)90975-7.
Complete cDNA copies of two variants of CARNA 5, the satellite of cucumber mosaic virus, have been cloned in the transcription vector pPM1. These two naturally-occurring CARNA 5s are capable and incapable, respectively, of inducing a lethal necrotic disease of tomato upon coinoculation with the genomic RNAs 1, 2, and 3 of cucumber mosaic virus. Uncapped transcripts synthesized in vitro from the two linearized, recombinant plasmids are infectious and each induces the appropriate symptomatology upon coinfection with cucumber mosaic viral RNAs on tomato plants. Progeny CARNA 5s isolated from such infected plants correspond to their natural CARNA 5 counterparts.
黄瓜花叶病毒卫星RNA CARNA 5的两个变体的完整cDNA拷贝已克隆到转录载体pPM1中。这两种天然存在的CARNA 5在与黄瓜花叶病毒的基因组RNA 1、2和3共同接种时，分别能够和不能够诱导番茄的致死性坏死病。从两个线性化的重组质粒体外合成的无帽转录本具有感染性，并且在与番茄植株上的黄瓜花叶病毒RNA共同感染时，每个转录本都会诱导相应的症状。从这种受感染植物中分离出的子代CARNA 5与它们天然的CARNA 5对应物一致。
