Mphahlele Matlale Phriskey, Oguttu James Wabwire, Petzer Inge-Marie, Qekwana Daniel Nenene
Section Veterinary Public Health, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa.
Department of Agriculture and Animal Health, College of Agriculture and Environmental Science, University of South Africa, Roodepoort, South Africa.
Vet World. 2020 Dec;13(12):2736-2742. doi: 10.14202/vetworld.2020.2736-2742. Epub 2020 Dec 21.
infections and antimicrobial resistance (AMR) in mastitis cases are both of clinical and economic importance. This study investigated the prevalence and AMR patterns of isolated from composite milk samples of dairy cows submitted to the Onderstepoort Milk Laboratory for routine diagnosis.
A total of 2862 cow milk samples randomly selected from submitted samples were tested for the presence of using microbiological and biochemical tests. Confirmation of isolates was done using the analytical profile index. Antimicrobial susceptibility of isolates against 12 antimicrobial agents was determined using the disk diffusion method.
was isolated from 1.7% (50/2862) of the samples tested. All (100%) isolates were resistant to at least one antimicrobial, while 62% (31/50) were resistant to three or more categories of antimicrobials (multidrug-resistant [MDR]). Most isolates were resistant to erythromycin (62%; 31/50) and ampicillin (62%; 31/50). Almost half of isolates were resistant to oxacillin (46%; 23/50) and only 8% (4/50) were resistant to cefoxitin.
Although the prevalence of among mastitis cases in this study was low, isolates exhibited high resistance to aminoglycosides, macrolides, and penicillins, all of which are important drugs in human medicine. The high prevalence of MDR and the presence of methicillin resistance among observed in this study are of both clinical and public health concerns.
乳腺炎病例中的感染及抗菌药物耐药性（AMR）在临床和经济方面均具有重要意义。本研究调查了提交至翁德斯特普特牛奶实验室进行常规诊断的奶牛复合乳样中分离出的[病原体名称未给出]的流行情况及AMR模式。
从提交的样本中随机选取共2862份牛奶样本，通过微生物学和生化检测来检测[病原体名称未给出]的存在情况。使用分析谱指数对分离株进行确认。采用纸片扩散法测定分离株对12种抗菌药物的药敏情况。
在所检测的样本中，1.7%（50/2862）分离出了[病原体名称未给出]。所有（100%）[病原体名称未给出]分离株对至少一种抗菌药物耐药，而62%（31/50）对三类或更多类抗菌药物耐药（多重耐药[MDR]）。大多数[病原体名称未给出]分离株对红霉素（62%；31/50）和氨苄西林（62%；31/50）耐药。几乎一半的[病原体名称未给出]分离株对苯唑西林耐药（46%；23/50），仅8%（4/50）对头孢西丁耐药。
尽管本研究中乳腺炎病例中[病原体名称未给出]的流行率较低，但分离株对氨基糖苷类、大环内酯类和青霉素类表现出高度耐药性，而这些在人类医学中都是重要药物。本研究中观察到的[病原体名称未给出]多重耐药的高流行率以及耐甲氧西林情况在临床和公共卫生方面均令人担忧。
