Department of Gastroenterology, Liaocheng People's Hospital, Liaocheng 252000, China.
Clinical Laboratory, Liaocheng People's Hospital, Liaocheng 252000, China.
ScientificWorldJournal. 2021 Jan 4;2021:3152304. doi: 10.1155/2021/3152304. eCollection 2021.
To study the effects of z-guggulsterone on gastric cancer cell apoptosis and the mechanism related.
Human gastric tumor SGC-7901 cells and GES-1 normal epithelial cells were treated with z-guggulsterone (0-75 M) for 24 h. MTT assay was applied to evaluate cell proliferation. Flow cytometry and Hoechst staining were used to assess cell apoptosis. Western blotting was applied to evaluate FXR, small heterodimer partner (SHP), Bcl-2, and Bax protein expression. ELISA was applied to gain the levels of active caspase-3 and the contents of TNF-, TGF-1, and VEGF.
The expression levels of FXR and SHP were higher in tumor cells than in normal epithelial cells. Inhibition of FXR signaling with z-guggulsterone dose-dependently inhibited SGC-7901 cell proliferation and promoted SGC-7901 cell apoptosis. Bcl-2 protein expression was significantly decreased, and active caspase-3 and Bax protein expression was increased in SGC-7901 cells incubated with z-guggulsterone. The content of TNF- was significantly increased, and the contents of VEGF and TGF-1 were decreased in SGC-7901 cells incubated with z-guggulsterone.
Inhibition of FXR signaling with z-guggulsterone induced anticancer effects in SGC-7901 cells by decreasing cell proliferation and promoting apoptosis. Z-guggulsterone induced cell apoptosis through the mitochondria-dependent pathway.
研究 Z-乳香酸对胃癌细胞凋亡的影响及其相关机制。
用 Z-乳香酸（0-75μM）处理人胃肿瘤 SGC-7901 细胞和 GES-1 正常上皮细胞 24 小时。MTT 法评估细胞增殖。流式细胞术和 Hoechst 染色评估细胞凋亡。Western blot 法评估 FXR、小异二聚体伴侣（SHP）、Bcl-2 和 Bax 蛋白表达。ELISA 法检测活性 caspase-3 和 TNF-、TGF-1、VEGF 的含量。
肿瘤细胞中 FXR 和 SHP 的表达水平高于正常上皮细胞。用 Z-乳香酸抑制 FXR 信号通路，呈剂量依赖性抑制 SGC-7901 细胞增殖并促进 SGC-7901 细胞凋亡。Bcl-2 蛋白表达显著降低，用 Z-乳香酸孵育的 SGC-7901 细胞中活性 caspase-3 和 Bax 蛋白表达增加。TNF-的含量显著增加，用 Z-乳香酸孵育的 SGC-7901 细胞中 VEGF 和 TGF-1 的含量降低。
用 Z-乳香酸抑制 FXR 信号通路通过降低细胞增殖和促进细胞凋亡来诱导 SGC-7901 细胞的抗癌作用。Z-乳香酸通过线粒体依赖的途径诱导细胞凋亡。
