Sapkota Sanjeep, Khadka Sujan, Adhikari Sanjib, Parajuli Ashish, Kandel Hemraj, Regmi Ramesh Sharma
Department of Microbiology, Birendra Multiple Campus, Tribhuvan University, Bharatpur, Chitwan, Nepal.
State Key Laboratory of Respiratory Disease, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
Int J Microbiol. 2020 Oct 29;2020:8877200. doi: 10.1155/2020/8877200. eCollection 2020.
Motorcycle helmets can serve as a potential vehicle for the transmission of pathogenic bacteria and fungi with serious health implications. The main aim of this study was to explore the microbial diversity associated with the motorcycle helmets and determine the antibiotic susceptibility profile of the bacterial isolates.
A descriptive cross-sectional study was carried out among the teaching staffs of Birendra Multiple Campus, Bharatpur, Nepal. A total of 130 motorcycle helmets worn by the teaching staffs of the Birendra Multiple Campus, Bharatpur, were included in the study for microbiological investigations.
Of the total 130 motorcycle helmets analyzed, 392 bacteria and 346 fungi belonging to seven different genera were recovered. 89 (22.7%) was the predominant bacteria followed by 77 (19.6%) and 54 (13.8%), whereas 67 (19.4%) was the predominant fungi followed by 49 (14.2%). Antibiotic susceptibility test was performed by the disc diffusion method for all the bacterial isolates. Tetracycline, gentamycin, and cotrimoxazole were the most effective antibiotics for Gram-positive isolates, whereas Gram-negative isolates were sensitive towards imipenem and ciprofloxacin. Of the total bacterial isolates, 153 (39.0%) were multidrug-resistant (MDR), 10.4% were extended-spectrum beta-lactamase (ESBL) producers, and 4.3% were metallo-beta-lactamase (MBL) producers and, out of 89 isolates of 30 (33.7%) were detected as methicillin-resistant (MRSA).
The findings suggest that motorcycle riders should follow good hygiene practices and regularly clean their helmets with suitable sterilants to avoid the risk of microbial contamination and reduce the associated risks.
摩托车头盔可能成为致病细菌和真菌传播的潜在媒介，对健康有严重影响。本研究的主要目的是探索与摩托车头盔相关的微生物多样性，并确定分离出的细菌的抗生素敏感性概况。
在尼泊尔巴拉特普尔比伦德拉多校区的教职员工中开展了一项描述性横断面研究。比伦德拉多校区教职员工佩戴的总共130顶摩托车头盔被纳入该研究进行微生物学调查。
在分析的130顶摩托车头盔中，共分离出属于七个不同属的392株细菌和346株真菌。89株（22.7%）是主要细菌，其次是77株（19.6%）和54株（13.8%），而67株（19.4%）是主要真菌，其次是49株（14.2%）。对所有分离出的细菌进行了纸片扩散法抗生素敏感性试验。四环素、庆大霉素和复方新诺明对革兰氏阳性菌分离株最有效，而革兰氏阴性菌分离株对亚胺培南和环丙沙星敏感。在所有细菌分离株中，153株（39.0%）为多重耐药（MDR），10.4%为超广谱β-内酰胺酶（ESBL）产生菌，4.3%为金属β-内酰胺酶（MBL）产生菌，在89株分离株中，30株（33.7%）被检测为耐甲氧西林金黄色葡萄球菌（MRSA）。
研究结果表明，摩托车骑行者应遵循良好的卫生习惯，定期用合适的消毒剂清洁头盔，以避免微生物污染风险并降低相关风险。
