Pala Sehmus, Atilgan Remzi, Kuloglu Tuncay, Yalçın Emre, Kaya Nalan, Etem Ebru
Department of Obstetrics and Gynecology, School of Medicine, Firat University, Elazig, Turkey.
Department of Histology and Embryology, School of Medicine, Firat University, Elazig, Turkey.
Arch Med Sci. 2019 Mar 19;17(1):228-235. doi: 10.5114/aoms.2019.83760. eCollection 2021.
The aim of the study was to investigate the association of transient receptor potential M2 (TRPM2) channel and muscarinic acetylcholine receptor 1 (CHRM1) activity with the memorial functions that are deteriorated in surgical menopause.
A total of 14 female rats were randomly divided into 2 groups: group (G)1: sham group; group (G)2: surgical menopause group, the group in which bilateral ovariectomy was performed. Fourteen days after the surgical procedure, learning and memorial tests were performed in G1 and G2 for a totally 13 days. The time required for the rats to find the cheese in the labyrinth was recorded and statistical evaluation of it was performed between groups. On the 14 day of the memory test, the rats were decapitated and the brain tissues were fixed in 10% formalin. Hippocampal TRPM2 and CHRM1 gene expression was evaluated with RNA isolation, complementary DNA (cDNA) synthesis and quantitative real-time PCR (qRT-PCR) analysis. TRPM2 and CHRM1 immunoreactivity was evaluated in hippocampal tissue with the immunohistochemical method. Histo-score was calculated regarding the diffuseness of and severity of the staining; and statistical analyses were performed.
In the ovariectomized group, the mean time required for the rats to find the cheese was statistically significantly elongated (39.29 ±4.0 s vs. 29.86 ±2.6 s). When the hippocampal TRPM2 and CHRM1 gene expression and immunoreactivity were compared with the sham group, there was a statistically significant decrease in the surgical menopause group ( < 0.05).
In surgical menopause, in deterioration of memorial functions, hippocampal TRPM2 channel and CHRM1 activity plays an important role.
本研究旨在探讨瞬时受体电位M2（TRPM2）通道和毒蕈碱型乙酰胆碱受体1（CHRM1）活性与手术绝经后受损的记忆功能之间的关联。
总共14只雌性大鼠被随机分为2组：第1组：假手术组；第2组：手术绝经组，即进行双侧卵巢切除术的组。手术操作14天后，在第1组和第2组中进行总共13天的学习和记忆测试。记录大鼠在迷宫中找到奶酪所需的时间，并在组间进行统计学评估。在记忆测试的第14天，将大鼠断头，脑组织固定于10%福尔马林中。通过RNA分离、互补DNA（cDNA）合成和定量实时聚合酶链反应（qRT-PCR）分析评估海马体中TRPM2和CHRM1基因表达。采用免疫组织化学方法评估海马组织中TRPM2和CHRM1的免疫反应性。计算关于染色的扩散程度和严重程度的组织评分；并进行统计学分析。
在卵巢切除组中，大鼠找到奶酪所需的平均时间在统计学上显著延长（39.29±4.0秒对29.86±2.6秒）。当将海马体中TRPM2和CHRM1基因表达及免疫反应性与假手术组进行比较时，手术绝经组有统计学显著下降（P<0.05）。
在手术绝经中，在记忆功能受损方面，海马体TRPM2通道和CHRM1活性起重要作用。
