Nguyen Phuong Thi Mai, Ngo Quang Van, Nguyen Minh Thi Hong, Quach Lien Thi, Pyne Stephen G
Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
Iran J Basic Med Sci. 2020 Dec;23(12):1558-1564. doi: 10.22038/ijbms.2020.46658.10763.
This research was carried out to investigate the hypoglycemic activity of the ethyl acetate (EtOAc) extract from the roots of Roxb, which strongly exhibit inhibitory activity against α-glucosidase and α-amylase on type 2 diabetic model.
Column chromatography combined with crystallization was used to isolate the active fraction and compounds. Chemical structures of the compounds were determined based on the analysis of the spectroscopic data and comparison with the literature data. The α-glucosidase inhibitory activity (AGI) and the α-amylase inhibitory activity (AAI) were determined quantitatively spectrophotometrically using p-nitrophenyl α-D-glucopyranoside and soluble starch as substrates, respectively. The hypoglycemic activity was examined by evaluating its effects on glucose and insulin levels, insulin resistance, and histopathology of the pancreatic islets and livers in diabetic induced mice administrated with nicotinamide-streptozotocin.
The EtOAc extract and the bioactive compounds astilbin and 5-O-caffeoylshikimic acid in the extract were isolated and confirmed in structures, AGI, and AAI. The treatment at the doses of 500 and 1000 µg/kg of body weight reduced blood glucose levels down to the physiological level of the physical controls in the diabetic mice after two weeks (<0.05). Moreover, the treatment improved insulin sensitivity. Histopathology analysis showed recovering effects in the size of the pancreatic islets and no damaging effects on the liver after treatment compared with the control group.
Our data suggest that the EtOAc extract possesses hypoglycemic activity and has an antidiabetic potential for therapeutic applications.
本研究旨在调查来自Roxb根的乙酸乙酯（EtOAc）提取物的降血糖活性，该提取物在2型糖尿病模型中对α-葡萄糖苷酶和α-淀粉酶具有强烈的抑制活性。
采用柱色谱结合结晶法分离活性成分和化合物。基于光谱数据分析并与文献数据比较确定化合物的化学结构。分别以对硝基苯基α-D-吡喃葡萄糖苷和可溶性淀粉为底物，采用分光光度法定量测定α-葡萄糖苷酶抑制活性（AGI）和α-淀粉酶抑制活性（AAI）。通过评估其对烟酰胺-链脲佐菌素诱导的糖尿病小鼠的血糖和胰岛素水平、胰岛素抵抗以及胰岛和肝脏组织病理学的影响来检测降血糖活性。
分离出EtOAc提取物以及提取物中的生物活性化合物落新妇苷和5-O-咖啡酰基莽草酸，并对其结构、AGI和AAI进行了确认。体重剂量为500和1000μg/kg的处理在两周后使糖尿病小鼠的血糖水平降至正常对照组的生理水平（<0.05）。此外，该处理改善了胰岛素敏感性。组织病理学分析表明，与对照组相比，处理后胰岛大小有恢复作用，且对肝脏无损伤作用。
我们的数据表明，EtOAc提取物具有降血糖活性，具有用于治疗的抗糖尿病潜力。
