Dallagnol Andrea Micaela, Pescuma Micaela, Espínola Natalia Gamarra, Vera Mariela, Vignolo Graciela Margarita
Instituto de Materiales de Misiones (IMAM), Facultad de Ciencias Exactas, Químicas y Naturales (FCEQyN), CONICET, UNaM, (Felix de Azara 1552), Posadas, Argentina.
Laboratorio de Microbiología de Alimentos y Biotecnología "Dr. Fernando O. Benassi", UNaM, (Ruta 12, Km 7.5), Posadas, Argentina.
Biotechnol Rep (Amst). 2021 Jan 6;29:e00589. doi: 10.1016/j.btre.2021.e00589. eCollection 2021 Mar.
Lactic acid bacteria (LAB) isolated from freshwater fish (hatcheries and captures) from Paraná river (Argentina) were analyzed by using culture-dependent approaches. The species belonging to (.) , . , . , . and (.) were identify as predominant by RAPD-PCR and 16 s rRNA gene sequencing. (H-17, S-30, B-42 and S-44) grew in raw fish extract and slightly reduced the medium pH (5.81-5.91). These strains exhibited moderate fish sarcoplasmic protein degradation (≤ 73 %) releasing small peptides and free amino acids, being alanine, glycine, asparagine and arginine concentrations increased in a higher extent (17.84, 1.47, 1.26 and 0.47 mg/100 mL, respectively) by S-44 strain at 96 h incubation. Interestingly H-17 was able to inhibit Results suggest that these strains would contribute to the development of new safe and healthy fishery products with improved nutritional and sensory characteristics.
采用基于培养的方法对从阿根廷巴拉那河的淡水鱼(孵化场和捕捞的鱼)中分离出的乳酸菌(LAB)进行了分析。通过随机扩增多态性DNA聚合酶链反应(RAPD-PCR)和16 s核糖体RNA(rRNA)基因测序,确定属于(.)、.、.、.和(.)的物种为优势种。(H-17、S-30、B-42和S-44)在生鱼提取物中生长,并使培养基pH值略有降低(5.81-5.91)。这些菌株表现出适度的鱼肉肌浆蛋白降解(≤73%),释放出小肽和游离氨基酸,在96小时培养时,S-44菌株使丙氨酸、甘氨酸、天冬酰胺和精氨酸的浓度升高幅度更大(分别为17.84、1.47、1.26和0.47毫克/100毫升)。有趣的是,H-17能够抑制……结果表明,这些菌株将有助于开发具有改善营养和感官特性的新型安全健康渔业产品。