Ohki Takeshi, Yamamoto Masakazu
Department of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan.
Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan.
Regen Ther. 2020 May 15;13:8-17. doi: 10.1016/j.reth.2020.04.009. eCollection 2020 Mar.
We have been conducting research on esophageal regenerative therapy using cell sheet technology. In particular, in the endoscopic field, we have pushed forward clinical research on endoscopic transplantation of cultured autologous oral mucosal epithelial cell sheets to esophageal ulcer after endoscopic submucosal dissection (ESD). We started research in this direction using animal models in 2004 and performed clinical research in 2012 in collaboration with Nagasaki University and Karolinska Institute. Although in full-circumferential cases it was difficult to prevent esophageal stricture after ESD, there were no complications and stricture could be suppressed. The cell sheet technology is still in its infancy. However, we are convinced that it has a high potential for application in various areas of gastrointestinal science. In this review, we focus on the pre-clinical and clinical trial results obtained and on the theoretical aspects of (1) stricture prevention, (2) esophageal tissue engineering research, and (3) endoscopic transplantation, and review the esophageal regenerative therapy by cell sheet technology.
我们一直在进行利用细胞片技术的食管再生治疗研究。特别是在内镜领域，我们推进了关于在内镜黏膜下剥离术（ESD）后将培养的自体口腔黏膜上皮细胞片内镜移植到食管溃疡的临床研究。我们于2004年开始使用动物模型朝这个方向开展研究，并于2012年与长崎大学和卡罗林斯卡学院合作进行了临床研究。尽管在全周病例中，ESD后难以预防食管狭窄，但未出现并发症且狭窄可得到抑制。细胞片技术仍处于起步阶段。然而，我们坚信它在胃肠科学的各个领域具有很高的应用潜力。在这篇综述中，我们重点关注所获得的临床前和临床试验结果以及（1）狭窄预防、（2）食管组织工程研究和（3）内镜移植的理论方面，并通过细胞片技术对食管再生治疗进行综述。
