Murphy E, Gabel S A, Funk A, London R E
Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Biochemistry. 1988 Jan 26;27(2):526-8. doi: 10.1021/bi00402a003.
The extent to which cellular metabolites are NMR observable is of fundamental importance in the interpretation of in vivo NMR studies. Analysis of ischemic rat liver shows that ATP resonances measured by 31P NMR decrease considerably faster than total tissue ATP measured in extracts. This discrepancy demonstrates that, in liver, ATP is not 100% observable. Furthermore, the data are consistent with the supposition that in situ mitochondrial ATP resonances are not normally observable by in vivo NMR techniques. The specificity of the NMR measurement for cytosolic ATP indicates that 31P NMR can be a valuable tool for the specific measurement of ATP in this compartment.
细胞代谢物的核磁共振（NMR）可观测程度在体内NMR研究的解释中至关重要。对缺血大鼠肝脏的分析表明，用31P NMR测量的ATP共振信号下降速度比提取物中测量的总组织ATP快得多。这种差异表明，在肝脏中，ATP并非100%可观测到。此外，这些数据与以下假设一致，即原位线粒体ATP共振信号通常无法通过体内NMR技术观测到。NMR对胞质ATP测量的特异性表明，31P NMR可成为特异性测量该区域ATP的有价值工具。
