Alazemi Ameerah M, Jamal W, Al Khabbaz A, Rotimi V O
Microbiology Department, Faculty of Medicine, Kuwait University, Kuwait.
Department of Surgical Sciences, Faculty of Dentistry, Kuwait University, Kuwait.
Access Microbiol. 2020 Nov 16;2(12):acmi000177. doi: 10.1099/acmi.0.000177. eCollection 2020.
Periodontal diseases are a group of chronic infections that destroy tissues surrounding and supporting the teeth. Data on the anaerobes associated with periodontal infections in Kuwait is lacking.
To investigate the target anaerobes associated with chronic periodontitis (CP) in patients admitted to Dental Clinics in Kuwait University Health Sciences Center, Kuwait.
Patients with CP (severe and moderate) were recruited into this study during a period of 15 months. Samples were collected directly from inside the gingival pockets and subjected to semi-quantitative PCR assays.
A total of 30 patients, stratified into moderate and severe CP and 31 healthy individuals, used as controls, were studied. Nine (30 %) of the 30 patients were in the 50-59-year age group. The detection rate of between the patients (9 : 30 %) versus the controls (5 : 16.1 %) was non-significant (0.05). spp., were detected in all patients versus 29 (93.1 %) controls, (0.05). However, four target anaerobes were significantly associated with CP patients; was detected in ten (33.3 %) patients versus two (6.4 %) controls ( <0.0001); 25 (83.3 %) versus 16 (51.6 %) controls ( <0.0001); 27 (90 %) versus 16 (51.6 %) controls ( <0.0001) and 18 (60 %) versus nine (29 %) controls ( <0.0001), respectively. spp. were detected in 27 (90 %) patients and 30 (96.7 %) controls (>0.5). There was no significant difference in the burden of spp. between patients and controls determined by semi-quantitative PCR assays.
Some (4/7) of the target anaerobes were significantly associated with CP in our study. was the most strongly associated anaerobe with CP, although not the keystone bacteria, while spp. was similar to the healthy controls.
牙周疾病是一组破坏牙齿周围和支持组织的慢性感染性疾病。科威特缺乏与牙周感染相关的厌氧菌数据。
调查科威特大学健康科学中心牙科诊所收治的慢性牙周炎（CP）患者中与之相关的目标厌氧菌。
在15个月的时间里招募了患有CP（重度和中度）的患者参与本研究。样本直接从牙龈袋内采集，并进行半定量PCR检测。
共研究了30例患者，分为中度和重度CP组，以及31名健康个体作为对照。30例患者中有9例（30%）年龄在50 - 59岁之间。患者组（9/30）与对照组（5/16.1）的检出率无显著差异（P>0.05）。所有患者均检测到……菌，而对照组为29例（93.1%），差异无统计学意义（P>0.05）。然而，四种目标厌氧菌与CP患者显著相关；……菌在10例（33.3%）患者中检出，而对照组为2例（6.4%）（P<0.0001）；……菌在25例（83.3%）患者中检出，对照组为16例（51.6%）（P<0.0001）；……菌在27例（90%）患者中检出，对照组为16例（51.6%）（P<0.0001）；……菌在18例（60%）患者中检出，对照组为9例（29%）（P<0.0001）。……菌在27例（90%）患者和30例（96.7%）对照组中检出（P>0.5）。通过半定量PCR检测，患者和对照组之间……菌的负荷无显著差异。
在我们的研究中，部分（4/7）目标厌氧菌与CP显著相关。……菌是与CP关联最密切的厌氧菌，尽管它不是关键细菌，而……菌与健康对照组相似。
