Laboratory of Oral Infection and Immunology, Institute of Stomatology, Nanjing Medical University, Nanjing, Jiang Su, China.
BMC Oral Health. 2014 Apr 13;14:37. doi: 10.1186/1472-6831-14-37.
Aggregatibacter actinomycetemcomitans (A.actinomycetemcomitans) is an important periodontal pathogen that can participate in periodontitis and other non-oral infections. The cytolethal distending toxin (Cdt) is among the virulence factors produced by this bacterium. This study was to elucidate the distribution of A.actinomycetemcomitans and the prevalence of its cdtB gene in Chinese subjects.
A total of 255 subgingival samples were obtained from 30 subjects. Samples were collected from periodontal healthy sites as well as shallow, moderate and deep pockets. The absolute quantity of A.actinomycetemcomitans and cdtB gene were determined by real-time polymerase chain reaction.
A.actinomycetemcomitans was detected in 92 of 105 (87.6%) samples of aggressive periodontitis (AgP) patients, in 73 of 79 (92.4%) samples of chronic periodontitis ( CP) patients and in 5 of 71 (7.0%) samples of periodontal healthy subjects. The cdtB gene was detected in 72 sites (78.3%) with AgP infected with A.actinomycetemcomitans, 54 sites (74.0%) with CP infected with A.actinomycetemcomitans and none in healthy sites infected with A.actinomycetemcomitans. In addition, quantity of A.actinomycetemcomitans and cdt gene in samples from deep pockets were significant larger than moderate, shallow and healthy sites (P < 0.05). In comparison to CP, AgP patients were infected with increased numbers of cdt genotype in deep pockets (P < 0.05).
This study suggests that the cdtB gene are prevalent in A.actinomycetemcomitans, and the distribution of cdt genotype strain may be correlated with AgP and serious periodontal inflammation.
伴放线放线杆菌（A. actinomycetemcomitans）是一种重要的牙周致病菌，它可以参与牙周炎和其他非口腔感染。细胞致死扩张毒素（Cdt）是该细菌产生的毒力因子之一。本研究旨在阐明中国人群中伴放线放线杆菌的分布及其 cdtB 基因的流行情况。
共从 30 名受试者中获得 255 个龈下样本。样本采集自牙周健康部位以及浅、中、深牙周袋。通过实时聚合酶链反应测定 A. actinomycetemcomitans 的绝对数量和 cdtB 基因。
侵袭性牙周炎（AgP）患者的 105 个样本中有 92 个（87.6%）检测到 A. actinomycetemcomitans，慢性牙周炎（CP）患者的 79 个样本中有 73 个（92.4%）检测到 A. actinomycetemcomitans，牙周健康受试者的 71 个样本中有 5 个（7.0%）检测到 A. actinomycetemcomitans。在 AgP 感染伴放线放线杆菌的 72 个部位（78.3%）、CP 感染伴放线放线杆菌的 54 个部位（74.0%）和牙周健康部位（均未感染伴放线放线杆菌）检测到 cdtB 基因。此外，深牙周袋中 A. actinomycetemcomitans 的数量和 cdt 基因的数量明显大于中牙周袋、浅牙周袋和牙周健康部位（P < 0.05）。与 CP 相比，AgP 患者深牙周袋中感染的 cdt 基因型数量增加（P < 0.05）。
本研究表明 cdtB 基因在伴放线放线杆菌中普遍存在，cdt 基因型菌株的分布可能与 AgP 和严重牙周炎症有关。
