Beach D A, Bustamante C, Wells K S, Foucar K M
Department of Chemistry, University of New Mexico, Albuquerque.
Biophys J. 1988 Mar;53(3):449-56. doi: 10.1016/S0006-3495(88)83121-7.
In this paper we test the predictions of the differential polarization imaging theory developed in the previous two papers. A characterization of the patterns of polymerization of hemoglobin in red blood cells from patients with sickle cell anemia is presented. This system was chosen because it is relatively easy to handle and because previous studies have been done on it. A differential polarization microscope designed and built in our laboratory was used to carry out this study. This microscope uses an image dissector camera, a photoelastic modulator, and a phase-lock amplifier. This design represents a substantial modification with respect to the instrumentation used in the previous results communicated on this system. Therefore, the results presented here also permit us to confirm the validity of our conclusions. On the basis of the differential polarization images obtained, models of the patterns of polymerization of the hemoglobin S inside the sickle cells are proposed and their M12 and regular images are calculated by the theory. Good agreement between those models and the experimental systems is found, as well as with the results previously reported.
在本文中，我们对前两篇论文中所提出的微分偏振成像理论的预测进行了检验。本文给出了镰状细胞贫血患者红细胞中血红蛋白聚合模式的特征描述。之所以选择这个系统，是因为它相对易于操作，且此前已有相关研究。我们使用在实验室设计并搭建的一台微分偏振显微镜来开展这项研究。这台显微镜采用了析像管相机、光弹性调制器和锁相放大器。相较于之前报道该系统的研究中所使用的仪器设备，这种设计有了实质性的改进。因此，本文所呈现的结果也使我们能够确认我们结论的有效性。基于所获得的微分偏振图像，我们提出了镰状细胞内血红蛋白S聚合模式的模型，并根据该理论计算出了它们的M12图像和常规图像。结果发现，这些模型与实验系统以及先前报道的结果均吻合良好。
