Beach D A, Bustamante C, Wells K S, Foucar K M
Department of Chemistry, University of New Mexico, Albuquerque 87131.
Biophys J. 1987 Dec;52(6):947-54. doi: 10.1016/S0006-3495(87)83287-3.
In this paper we test the predictions of the differential polarization imaging theory developed in the previous two papers. A characterization of the patterns of polymerization of hemoglobin in red blood cells from patients with sickle cell anemia is presented. This system was chosen because it is relatively easy to handle and because previous studies have been done on it. A differential polarization microscope designed and built in our laboratory was used to carry out this study. This microscope uses an image dissector camera, a photoelastic modulator, and a phase-lock amplifier. This design represents a substantial modification with respect to the instrumentation used in the previous results communicated on this system. Therefore, the results presented here also permit us to confirm the validity of our conclusions. On the basis of the differential polarization images obtained, models of the patterns of polymerization of the hemoglobin S inside the sickle cells are proposed and their M12 and regular images are calculated by the theory. Good agreement between those models and the experimental systems is found, as well as with the results previously reported.
在本文中，我们对在前两篇论文中所提出的微分偏振成像理论的预测进行了检验。给出了镰状细胞贫血患者红细胞中血红蛋白聚合模式的特征描述。选择这个系统是因为它相对易于操作，并且之前已经对其进行过研究。我们使用在实验室设计并制造的微分偏振显微镜来开展这项研究。该显微镜使用了图像析像管相机、光弹性调制器和锁相放大器。相对于在之前关于该系统所报道的结果中所使用的仪器，这种设计有了实质性的改进。因此，这里呈现的结果也使我们能够确认我们结论的有效性。基于所获得的微分偏振图像，提出了镰状细胞内血红蛋白S聚合模式的模型，并通过该理论计算出它们的M12和常规图像。发现这些模型与实验系统之间以及与之前报道的结果之间都有很好的一致性。
