单个红细胞内镰状血红蛋白聚合物解聚动力学的光散射研究
Light-scattering study of depolymerization kinetics of sickle hemoglobin polymers inside single erythrocytes.
作者信息
Peetermans J, Nishio I, Ohnishi S T, Tanaka T
出版信息
Proc Natl Acad Sci U S A. 1986 Jan;83(2):352-6. doi: 10.1073/pnas.83.2.352.
Abstract
The time course of intracellular depolymerization of hemoglobin S aggregates is directly observed by using microscope laser light-scattering spectroscopy in single sickle erythrocytes upon slow reoxygenation. From the correlation functions of the light intensity scattered from a single cell, we determine the average diffusion coefficient as well as the fraction of hemoglobin aggregates that have intracellular mobility. The oxygen saturation of the hemoglobin of the same cell is measured by single-cell absorption spectrophotometry. Combining the results obtained with these techniques and information on the cellular morphology, we propose a model for the depolymerization process of hemoglobin inside single erythrocytes as they transform from the fully sickled to the normal biconcave shape upon reoxygenation.
摘要
在缓慢复氧过程中，通过显微镜激光散射光谱法直接观察单个镰状红细胞内血红蛋白S聚集体的细胞内解聚时间进程。从单个细胞散射光强度的相关函数中，我们确定了平均扩散系数以及具有细胞内流动性的血红蛋白聚集体的比例。通过单细胞吸收分光光度法测量同一细胞内血红蛋白的氧饱和度。结合这些技术获得的结果以及细胞形态学信息，我们提出了一个模型，用于描述单个红细胞内血红蛋白在复氧过程中从完全镰状形态转变为正常双凹形态时的解聚过程。
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