Department of Advanced Technology and Biology, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Pde, Parkville, Victoria 3052, Australia.
Department of Medical Biology, The University of Melbourne, Royal Pde, Parkville, Victoria 3050, Australia.
Biochem Soc Trans. 2021 Feb 26;49(1):393-403. doi: 10.1042/BST20200688.
RNA-binding proteins are customarily regarded as important facilitators of gene expression. In recent years, RNA-protein interactions have also emerged as a pervasive force in the regulation of homeostasis. The compendium of proteins with provable RNA-binding function has swelled from the hundreds to the thousands astride the partnership of mass spectrometry-based proteomics and RNA sequencing. At the foundation of these advances is the adaptation of RNA-centric capture methods that can extract bound protein that has been cross-linked in its native environment. These methods reveal snapshots in time displaying an extensive network of regulation and a wealth of data that can be used for both the discovery of RNA-binding function and the molecular interfaces at which these interactions occur. This review will focus on the impact of these developments on our broader perception of post-transcriptional regulation, and how the technical features of current capture methods, as applied in mammalian systems, create a challenging medium for interpretation by systems biologists and target validation by experimental researchers.
RNA 结合蛋白通常被视为基因表达的重要促进因子。近年来，RNA-蛋白质相互作用也成为了内稳态调控的普遍力量。基于质谱的蛋白质组学和 RNA 测序的合作，具有可证明 RNA 结合功能的蛋白质的总数从数百个增加到数千个。这些进展的基础是 RNA 中心捕获方法的适应性，该方法可以提取在其天然环境中交联的结合蛋白。这些方法揭示了时间上的快照，显示了广泛的调控网络和丰富的数据，可用于发现 RNA 结合功能以及发生这些相互作用的分子界面。本综述将重点介绍这些发展对我们对转录后调控的更广泛认识的影响，以及当前捕获方法的技术特征如何在哺乳动物系统中应用，为系统生物学家的解释和实验研究人员的靶标验证带来挑战。
