Center for RNA Science and Therapeutics, School of Medicine, Case Western Reserve University, Cleveland, Ohio.
Wiley Interdiscip Rev RNA. 2020 Jan;11(1):e1565. doi: 10.1002/wrna.1565. Epub 2019 Aug 20.
RNA-protein interactions are pivotal for the regulation of gene expression from bacteria to human. RNA-protein interactions are dynamic; they change over biologically relevant timescales. Understanding the regulation of gene expression at the RNA level therefore requires knowledge of the dynamics of RNA-protein interactions. Here, we discuss the main experimental approaches to measure dynamic aspects of RNA-protein interactions. We cover techniques that assess dynamics of cellular RNA-protein interactions that accompany biological processes over timescales of hours or longer and techniques measuring the kinetic dynamics of RNA-protein interactions in vitro. This article is categorized under: RNA Interactions with Proteins and Other Molecules > Protein-RNA Interactions: Functional Implications RNA Interactions with Proteins and Other Molecules > Protein-RNA Recognition RNA Interactions with Proteins and Other Molecules > RNA-Protein Complexes RNA Evolution and Genomics > Ribonomics.
RNA-蛋白质相互作用对于从细菌到人类的基因表达调控至关重要。RNA-蛋白质相互作用是动态的；它们会在生物学相关的时间尺度上发生变化。因此，要了解 RNA 水平上的基因表达调控，就需要了解 RNA-蛋白质相互作用的动态特性。在这里，我们讨论了测量 RNA-蛋白质相互作用动态方面的主要实验方法。我们涵盖了评估伴随生物过程的细胞 RNA-蛋白质相互作用动力学的技术，这些过程的时间尺度为数小时或更长，以及测量 RNA-蛋白质相互作用在体外的动力学的技术。本文属于以下分类：RNA 与蛋白质和其他分子的相互作用 > 蛋白质-RNA 相互作用：功能意义 RNA 与蛋白质和其他分子的相互作用 > 蛋白质-RNA 识别 RNA 与蛋白质和其他分子的相互作用 > RNA-蛋白质复合物 RNA 进化和基因组学 > 核糖组学。
