Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing, China.
Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing, China.
Int J Biol Macromol. 2021 Mar 15;173:481-490. doi: 10.1016/j.ijbiomac.2021.01.139. Epub 2021 Jan 22.
In recent decades, the composition, structure, biosynthesis, and function of bacterial extracellular polysaccharides (EPS) have been extensively studied. EPS are synthesized through different biosynthetic pathways. The genes responsible for EPS synthesis are usually clustered on the genome or large plasmids of bacteria. Generally, different EPS synthesis gene clusters direct the synthesis of EPS with different chemical structures and biological activities. A better understanding of the gene functions involved in EPS biosynthesis is critical for the production of EPS with special biological activities. Genetic engineering methods are usually used to study EPS synthesis related genes. This review organizes the available information on EPS, including their structures, synthesis of related genes, and highlights the research progress of modifying EPS gene clusters through gene-editing methods.
近几十年来，人们对细菌胞外多糖（EPS）的组成、结构、生物合成和功能进行了广泛的研究。EPS 通过不同的生物合成途径合成。负责 EPS 合成的基因通常在细菌的基因组或大型质粒上聚集。通常，不同的 EPS 合成基因簇指导具有不同化学结构和生物活性的 EPS 的合成。更好地了解参与 EPS 生物合成的基因功能对于生产具有特殊生物活性的 EPS 至关重要。遗传工程方法通常用于研究 EPS 合成相关基因。本综述整理了 EPS 的相关信息，包括其结构、相关基因的合成，并重点介绍了通过基因编辑方法修饰 EPS 基因簇的研究进展。
