基于细胞和斑马鱼生物测定的不同灵芝孢子粉的抗肿瘤作用。
Antitumor effects of different Ganoderma lucidum spore powder in cell- and zebrafish-based bioassays.
机构信息
Zhejiang Shouxiangu Pharmaceutical Co., Ltd, Wuyi 321200, Zhejiang Province, China.
Zhejiang Shouxiangu Botanical Drug Institute Co., Ltd, Hangzhou 310012, Zhejiang Province, China.
出版信息
J Integr Med. 2021 Mar;19(2):177-184. doi: 10.1016/j.joim.2021.01.004. Epub 2021 Jan 13.
OBJECTIVE
Ganoderma lucidum spore (GLS) is gaining recognition as a medicinal part of G. lucidum and has been reported to possess various pharmacological properties, such as antitumor activity. In this work, wall-broken GLS powder (BGLSP) and wall-removed GLS powder (RGLSP), two kinds of GLS powder with different manufacturing techniques, were compared in terms of contents of active constituents and in vivo and in vitro antitumor effects.
METHODS
The ultraviolet and visible spectrophotometry method was used to determine the contents of polysaccharides and total triterpenoids in BGLSP and RGLSP. Seventeen individual triterpenoids were further quantified using ultra-high-performance liquid chromatography and quantitative analysis of multi-components by single marker. The antitumor effects of BGLSP and RGLSP were evaluated using in vitro cell viability assay against human gastric carcinoma SGC-7901, lung carcinoma A549 and lymphoma Ramos and further validated by in vivo zebrafish xenograft models with transplanted SGC-7901, A549 and Ramos.
RESULTS
The results showed that the contents of polysaccharides, total triterpenoids and individual triterpenoids of RGLSP were significantly higher than those of BGLSP. Although both BGLSP and RGLSP inhibited the three tumor cell lines in vitro in a dose-dependent manner, the inhibitory effects of RGLSP were much better than those of BGLSP. In the in vivo zebrafish assay, RGLSP exhibited more potent inhibitory activities against tumors transplanted into the zebrafish compared with BGLSP, and the inhibition rates of RGLSP reached approximately 78%, 31% and 83% on SGC-7901, A549 and Ramos, respectively.
CONCLUSION
The results indicated that the antitumor effects of GLS were positively correlated with the contents of the polysaccharides and triterpenoids and demonstrated that the wall-removing manufacturing technique could significantly improve the levels of active constituents, and thereby enhance the antitumor activity.
目的
灵芝孢子（GLS）作为灵芝的药用部位日益受到关注，已报道具有多种药理活性，如抗肿瘤活性。本研究比较了破壁灵芝孢子粉（BGLSP）和去壁灵芝孢子粉（RGLSP）这两种不同生产工艺的 GLS 粉，比较了它们的活性成分含量以及体内和体外抗肿瘤作用。
方法
采用紫外可见分光光度法测定 BGLSP 和 RGLSP 中多糖和总三萜的含量。采用超高效液相色谱法进一步定量测定 17 种单体三萜，并采用单标志物定量分析多组分法进行定量分析。采用体外细胞活力测定法评价 BGLSP 和 RGLSP 对人胃癌 SGC-7901、肺癌 A549 和淋巴瘤 Ramos 的抗肿瘤作用，并进一步通过 SGC-7901、A549 和 Ramos 移植的斑马鱼体内异种移植模型进行验证。
结果
结果表明，RGLSP 的多糖、总三萜和单体三萜含量明显高于 BGLSP。虽然 BGLSP 和 RGLSP 均能以剂量依赖的方式抑制三种肿瘤细胞系的体外生长，但 RGLSP 的抑制作用明显优于 BGLSP。在体内斑马鱼试验中，RGLSP 对移植到斑马鱼体内的肿瘤表现出更强的抑制活性，其抑制率对 SGC-7901、A549 和 Ramos 分别达到约 78%、31%和 83%。
结论
研究结果表明，GLS 的抗肿瘤作用与多糖和三萜含量呈正相关，表明去壁生产工艺可显著提高活性成分水平，从而增强抗肿瘤活性。
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