School of Perfume & Aroma and Cosmetics, Shanghai Institute of Technology, Shanghai 201418, China.
Engineering Research Center of Perfume & Aroma and Cosmetics, Ministry of Education, Shanghai 201418, China.
Molecules. 2024 May 16;29(10):2348. doi: 10.3390/molecules29102348.
spore powder, valued for its nutritional and medicinal properties, contains polysaccharides crucial for its efficacy. However, the complex structural nature of these polysaccharides necessitates further investigation to fully realize their potential. This study aimed to investigate the effects of acid heat treatment on spore polysaccharides (GLSPs) to enhance their properties and application in antitumor activity. The GLSP was obtained via acid heat treatment, concentration, and centrifugal separation. This process led to a notable reduction in polysaccharide molecular weight, increasing water solubility and bioavailability. Analytical techniques including NMR spectroscopy and methylation analysis revealed a polysaccharide composition comprising four distinct monosaccharides, with molecular weights of 3291 Da (Mw) and 3216 Da (Mn). Six different linkage modes were identified, with a molar ratio of 1:5:2:3:4:3. In vivo experiments demonstrated the GLSP's significant inhibitory effect on the growth of four tumor models (sarcoma S180, Lewis lung cancer, liver cancer H22, and colon cancer C26) in mice, with no observed toxicity. These findings suggest the GLSP's potential as an antitumor therapeutic agent for clinical use.
孢子粉因其营养和药用特性而备受重视，其中的多糖对于其功效至关重要。然而，这些多糖复杂的结构性质需要进一步研究，以充分发挥其潜力。本研究旨在探讨酸热处理对孢子多糖（GLSPs）的影响，以增强其性质并应用于抗肿瘤活性。通过酸热处理、浓缩和离心分离获得 GLSP。这一过程导致多糖分子量显著降低，提高了其水溶性和生物利用度。NMR 光谱和甲基化分析等分析技术揭示了多糖的组成，包含四种不同的单糖，分子量分别为 3291 Da（Mw）和 3216 Da（Mn）。鉴定出六种不同的键合模式，摩尔比为 1:5:2:3:4:3。体内实验表明，GLSP 对小鼠四种肿瘤模型（肉瘤 S180、Lewis 肺癌、肝癌 H22 和结肠癌 C26）的生长具有显著的抑制作用，且没有观察到毒性。这些发现表明 GLSP 有作为抗肿瘤治疗剂应用于临床的潜力。
