King M P, Attardi G
Division of Biology, California Institute of Technology, Pasadena 91125.
Cell. 1988 Mar 25;52(6):811-9. doi: 10.1016/0092-8674(88)90423-0.
Isolated human mitochondria containing a mitochondrial DNA (mtDNA) coded chloramphenicol resistance marker were injected into cells from two different human sensitive cell lines, 143BTK- and HT1080-6TG, which had been partially depleted of their mtDNA by ethidium bromide treatment. On the basis of the available evidence concerning the tolerance of introduced volumes into mammalian cells, it is estimated that, on the average, less than one mitochondrion was introduced into each cell. Under selective conditions, the mitochondria became established in the recipient cells with a frequency greater than 2-3 x 10(-3). An analysis of multiple mtDNA and nuclear DNA polymorphisms revealed a rapid replacement of the resident mtDNA by the exogenous mtDNA. Six to ten weeks after microinjection, this replacement was complete in all but one of the HT1080-6TG transformants, and nearly complete in the majority of the 143BTK- transformants. The quantitative behavior of the mtDNA of the transformants at very early stages of selection strongly suggests that intracellular mtDNA selection played a crucial role in this replacement, with significant implications for mitochondrial genetics.
将含有线粒体DNA（mtDNA）编码的氯霉素抗性标记的分离的人线粒体注射到来自两种不同的人敏感细胞系（143BTK-和HT1080-6TG）的细胞中，这两种细胞系已通过溴化乙锭处理部分耗尽其mtDNA。根据有关向哺乳动物细胞中引入体积耐受性的现有证据，估计平均每个细胞引入的线粒体少于一个。在选择条件下，线粒体在受体细胞中以大于2 - 3×10（-3）的频率建立。对多个mtDNA和核DNA多态性的分析表明，外源性mtDNA迅速取代了内源性mtDNA。显微注射后6至10周，除一个HT1080-6TG转化体之外，所有转化体中的这种取代均已完成，并且在大多数143BTK-转化体中几乎完成。在选择的非常早期阶段，转化体的mtDNA的定量行为强烈表明细胞内mtDNA选择在这种取代中起关键作用，这对线粒体遗传学具有重要意义。
