Sun Y, Oberley L W, Li Y
Department of Biochemistry, Zhejiang Medical University, Hangchow, The People's Republic of China.
Clin Chem. 1988 Mar;34(3):497-500.
This assay for superoxide dismutase (SOD, EC 1.15.1.1) activity involves inhibition of nitroblue tetrazolium reduction, with xanthine-xanthine oxidase used as a superoxide generator. By using a reaction terminator, we can determine 40 samples within 55 min. One unit of activity of pure bovine liver Cu,ZnSOD and chicken liver MnSOD was expressed by 30 ng and 500 ng of protein, respectively. The mean concentrations of Cu,ZnSOD as measured by this method in blood from normal adults were 242 (SEM 4) mg/L in erythrocytes, 548 (SEM 20) micrograms/L in serum, and 173 (SEM 11) micrograms/L in plasma. The Cu,ZnSOD concentrations in serum and plasma of patients with cancer of the large intestine tended to be less and greater than these values, respectively, but not statistically significantly so.
这种超氧化物歧化酶（SOD，EC 1.15.1.1）活性测定方法涉及抑制氮蓝四唑还原，以黄嘌呤 - 黄嘌呤氧化酶作为超氧化物生成剂。通过使用反应终止剂，我们可以在55分钟内测定40个样本。纯牛肝铜锌超氧化物歧化酶和鸡肝锰超氧化物歧化酶的一个活性单位分别由30 ng和500 ng蛋白质表示。用该方法测定的正常成年人血液中铜锌超氧化物歧化酶的平均浓度为：红细胞中242（标准误4）mg/L，血清中548（标准误20）μg/L，血浆中173（标准误11）μg/L。大肠癌症患者血清和血浆中的铜锌超氧化物歧化酶浓度分别倾向于低于和高于这些值，但差异无统计学意义。
