Department of Applied Chemistry, Dong-Eui University, Busan, South Korea.
Cell Biochem Funct. 2021 Jun;39(4):536-545. doi: 10.1002/cbf.3619. Epub 2021 Jan 25.
Melanin plays an important role in determining skin colour. Apoptosis of melanocytes and defect in melanin production cause vitiligo. Various studies have been conducted to treat the disease, but its treatment is still difficult. Therefore, this study aimed to investigate the effect of spermidine, which is known as an inhibitor of ageing-related oxidized proteins, on melanogenesis. Even though spermidine above 50 μM had no effect on antioxidant activity and DOPA oxidation, it displayed tyrosinase activity. However, spermidine at 2000 μM was cytotoxic in B16F1 cells using MTT assay. Spermidine above 125 μM decreased the amount of intracellular hydrogen peroxide in a concentration-dependent manner in DCFH-DA analysis. It was also found that spermidine above 2000 μM increased melanin synthesis in living cells. However, spermidine above 1000 μM increased melanin synthesis in a concentration-dependent manner in H O -treated B16F1 cells. Furthermore, spermidine enhanced the expression of tyrosine hydroxylase via MITF transcription factor involved in melanogenesis in H O -treated B16F1 cells. Therefore, these results suggest that spermidine could be applied as a potential stimulator of melanin synthesis for the prevention of hair greying.
黑色素在决定皮肤颜色方面起着重要作用。黑素细胞凋亡和黑色素生成缺陷导致白癜风。已经进行了各种研究来治疗这种疾病，但治疗仍然很困难。因此，本研究旨在探讨作为一种抑制与衰老相关的氧化蛋白的物质——亚精胺对黑色素生成的影响。尽管 50μM 以上的亚精胺对抗氧化活性和 DOPA 氧化没有影响，但它显示出了酪氨酸酶活性。然而，用 MTT 测定法在 B16F1 细胞中，2000μM 的亚精胺具有细胞毒性。在 DCFH-DA 分析中，亚精胺在 125μM 以上以浓度依赖的方式减少细胞内过氧化氢的量。还发现，2000μM 以上的亚精胺在活细胞中增加黑色素的合成。然而，在 H2O2 处理的 B16F1 细胞中，1000μM 以上的亚精胺以浓度依赖的方式增加黑色素的合成。此外，亚精胺通过参与黑色素生成的 MITF 转录因子增强了 H2O2 处理的 B16F1 细胞中酪氨酸羟化酶的表达。因此，这些结果表明，亚精胺可以作为一种潜在的黑色素合成刺激剂，用于预防头发变白。
