Shi Dian-Hua, Dai Yan-Peng, Su Ben-Zheng, Sun Li-Li, Zhang Xue-Lan, Zhang Jun
Shandong Academy of Chinese Medicine Ji'nan 250014, China Shandong University of Traditional Chinese Medicine Ji'nan 250355, China.
Shandong Academy of Chinese Medicine Ji'nan 250014, China.
Zhongguo Zhong Yao Za Zhi. 2020 Dec;45(24):5996-6002. doi: 10.19540/j.cnki.cjcmm.20200904.301.
In order to effectively solve the over-processing problem of Platycladi Cacumen Carbonisata, which was commonly used as a hemostatic drug in clinical application, we used the quantitative analysis method of multi-components by single marker(QAMS) in this study, with quercetin as internal reference to simultaneously determine the content of six flavonoids which can be used to control the internal quality of Platycladi Cacumen Carbonisata. Based on the comparison of QAMS and external standard method(ESM) results, the limit standards of contents were established as follows: isoquercitroside ≥0.002 0%, quercitroside ≥0.050%, quercetin ≥0.030%, kaempferol and amentoflavone both ≥0.010%, hinokiflavone ≥0.050%. Based on the color detection of Platycladi Cacumen and Platycladi Cacumen Carbonisata with different processing degrees, the law of influence of different processing degrees on the color of Platycladi Cacumen Carbonisata was found. A new external quality standard of Platycladi Cacumen Carbonisata was established by fitting curve of color recognition for the external quality control, based on which the standard ranges of ΔL*, Δb* and ΔE were-50.00--44.00, 6.00-11.00 and 45.00-50.00 respectively. Effective combination of established internal and external quality control standards by this study can be used to evaluate the processing degree and quality of Platycladi Cacumen Carbonisata more comprehensively and objectively, which can guarantee its clinical efficacy. At the same time, this study also provides reference and basis for further improving the quality control standard of Platycladi Cacumen Carbonisata.
为有效解决临床常用止血药侧柏炭的过度炮制问题，本研究采用一测多评法（QAMS），以槲皮素为内参，同时测定6种黄酮类成分的含量，用于控制侧柏炭的内在质量。通过比较QAMS与外标法（ESM）的结果，建立了含量限度标准如下：异槲皮苷≥0.002 0%，槲皮苷≥0.050%，槲皮素≥0.030%，山柰酚和穗花杉双黄酮均≥0.010%，扁柏双黄酮≥0.050%。通过对不同炮制程度的侧柏及侧柏炭进行颜色检测，发现了不同炮制程度对侧柏炭颜色的影响规律。通过颜色识别拟合曲线建立了侧柏炭的外观质量控制新的外在质量标准，基于此，ΔL*、Δb*和ΔE的标准范围分别为-50.00--44.00、6.00-11.00和45.00-50.00。本研究建立的内在和外在质量控制标准有效结合，可更全面、客观地评价侧柏炭的炮制程度和质量，保证其临床疗效。同时，本研究也为进一步完善侧柏炭的质量控制标准提供了参考和依据。
