血液中淋巴细胞亚群的直接免疫磁珠定量分析。
Direct immunomagnetic quantification of lymphocyte subsets in blood.
作者信息
Brinchmann J E, Vartdal F, Gaudernack G, Markussen G, Funderud S, Ugelstad J, Thorsby E
机构信息
Institute of Transplantation Immunology, National Hospital, Oslo, Norway.
出版信息
Clin Exp Immunol. 1988 Jan;71(1):182-6.
Abstract
A method is described where superparamagnetic polymer microspheres coated with monoclonal antibodies (MoAb) are used for the direct and fast quantification of the absolute number of cells of various lymphocyte subsets in blood. Blood samples were incubated with microspheres coated with a subset specific MoAb. Using a magnet the microsphere-rosetted cells were isolated and washed. Following lysis of the cell walls to detach the microspheres, the cell nuclei were stained with acridine orange and counted in a haemocytometer using an immunofluorescence microscope. With MoAb specific for CD2, CD4, CD8 and CD19, reproducible absolute counts of the corresponding lymphocyte subsets were obtained which correlated closely with those obtained by an indirect quantification method.
摘要
本文描述了一种方法，其中用单克隆抗体（MoAb）包被的超顺磁性聚合物微球用于直接快速定量血液中各种淋巴细胞亚群的绝对细胞数。血液样本与包被有亚群特异性MoAb的微球一起孵育。使用磁铁分离并洗涤微球玫瑰花结化的细胞。在裂解细胞壁以分离微球后，细胞核用吖啶橙染色，并使用免疫荧光显微镜在血细胞计数器中进行计数。使用针对CD2、CD4、CD8和CD19的MoAb，获得了相应淋巴细胞亚群的可重复绝对计数，这些计数与通过间接定量方法获得的计数密切相关。
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